• Confocal imaging of NIH/3T3 cells using PIK3R4/VPS15 Rabbit monoclonal antibody (STJ11103589, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Western blot analysis of lysates from Rat brain, using PIK3R4/VPS15 Rabbit monoclonal antibody (STJ11103589) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of various lysates using PIK3R4/VPS15 Rabbit monoclonal antibody (STJ11103589) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PIK3R4/VPS15 antibody (1259-1358) [S9MR] (STJ11103589)

SKU:
STJ11103589

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PIK3R4/VPS15 (1259-1358) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PIK3R4
Gene ID: 30849
Uniprot ID: PI3R4_HUMAN
Immunogen Region: 1259-1358
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1259-1358 of human PIK3R4/VPS15 (Q99570).
Immunogen Sequence: AGSDMKIRFWDLAYPERSYV VAGSTSSPSVSYYRKIIEGT EVVQEIQNKQKVGPSDDTPR RGPESLPVGHHDIITDVATF QTTQGFIVTASRDGIVKVWK
Tissue Specificity Ubiquitously expressed.
Post Translational Modifications Myristoylated. Probably autophosphorylated.
Function Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate.different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2.
Protein Name Phosphoinositide 3-Kinase Regulatory Subunit 4
Pi3-Kinase Regulatory Subunit 4
Pi3-Kinase P150 Subunit
Phosphoinositide 3-Kinase Adaptor Protein
Database Links Reactome: R-HSA-109704
Reactome: R-HSA-1632852
Reactome: R-HSA-1660514
Reactome: R-HSA-1660516
Reactome: R-HSA-1660517
Reactome: R-HSA-168138
Reactome: R-HSA-5668599
Reactome: R-HSA-9679504
Reactome: R-HSA-9694676
Reactome: R-HSA-9705671
Reactome: R-HSA-983170
Cellular Localisation Late Endosome
Cytoplasmic Vesicle
Autophagosome
Membrane
Lipid-Anchor
As Component Of The Pi3k Complex I Localized To Pre-Autophagosome Structures
As Component Of The Pi3k Complex Ii Localized Predominantly To Endosomes
Localizes Also To Discrete Punctae Along The Ciliary Axoneme
Alternative Antibody Names Anti-Phosphoinositide 3-Kinase Regulatory Subunit 4 antibody
Anti-Pi3-Kinase Regulatory Subunit 4 antibody
Anti-Pi3-Kinase P150 Subunit antibody
Anti-Phosphoinositide 3-Kinase Adaptor Protein antibody
Anti-PIK3R4 antibody
Anti-VPS15 antibody

Information sourced from Uniprot.org

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