• Western blot analysis of extracts of various cell lines, using PI3 Kinase p85 beta antibody (STJ11102171) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PIK3R2 antibody (100-200) [S1MR] (STJ11102171)

SKU:
STJ11102171

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PI 3 Kinase p85 beta (100-200) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PIK3R2
Gene ID: 5296
Uniprot ID: P85B_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human PI3 Kinase p85 beta (O00459).
Immunogen Sequence: PRDGAPEPGLTLPDLPEQFS PPDVAPPLLVKLVEAIERTG LDSESHYRPELPAPRTDWSL SDVDQWDTAALADGIKSFLL ALPAPLVTPEASAEARRALR E
Post Translational Modifications Phosphorylated in response to signaling from activated receptor-type protein kinases. Dephosphorylated by PTPRJ. Dephosphorylated at Tyr-655 by PTPN13. Phosphorylation of Tyr-655 impairs while its dephosphorylation promotes interaction with FBXL2 and SCF(FBXL2)-mediated polyubiquitination. Ubiquitinated. Polyubiquitination by the SCF(FBXL2) complex probably promotes proteasomal degradation of PIK3R2.
Function Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy. Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement.
Protein Name Phosphatidylinositol 3-Kinase Regulatory Subunit Beta
Pi3-Kinase Regulatory Subunit Beta
Pi3k Regulatory Subunit Beta
Ptdins-3-Kinase Regulatory Subunit Beta
Phosphatidylinositol 3-Kinase 85 Kda Regulatory Subunit Beta
Pi3-Kinase Subunit P85-Beta
Ptdins-3-Kinase Regulatory Subunit P85-Beta
Database Links Reactome: R-HSA-109704
Reactome: R-HSA-112399
Reactome: R-HSA-114604
Reactome: R-HSA-1257604
Reactome: R-HSA-1266695
Reactome: R-HSA-1433557
Reactome: R-HSA-1660499
Reactome: R-HSA-186763
Reactome: R-HSA-198203
Reactome: R-HSA-201556
Reactome: R-HSA-202424
Reactome: R-HSA-2029485
Reactome: R-HSA-210993
Reactome: R-HSA-2219530
Reactome: R-HSA-2424491
Reactome: R-HSA-2730905
Reactome: R-HSA-373753
Reactome: R-HSA-388841
Reactome: R-HSA-389357
Reactome: R-HSA-416476
Reactome: R-HSA-4420097
Reactome: R-HSA-512988
Reactome: R-HSA-5673001
Reactome: R-HSA-6811558
Reactome: R-HSA-8853659
Reactome: R-HSA-8980692
Reactome: R-HSA-9009391
Reactome: R-HSA-9013026
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013405
Reactome: R-HSA-9013409
Reactome: R-HSA-9013420
Reactome: R-HSA-9013423
Reactome: R-HSA-9035034
Reactome: R-HSA-912526
Reactome: R-HSA-912631
Reactome: R-HSA-9670439
Reactome: R-HSA-9673767
Reactome: R-HSA-9673770
Reactome: R-HSA-9696264
Reactome: R-HSA-9696270
Reactome: R-HSA-9696273
Alternative Antibody Names Anti-Phosphatidylinositol 3-Kinase Regulatory Subunit Beta antibody
Anti-Pi3-Kinase Regulatory Subunit Beta antibody
Anti-Pi3k Regulatory Subunit Beta antibody
Anti-Ptdins-3-Kinase Regulatory Subunit Beta antibody
Anti-Phosphatidylinositol 3-Kinase 85 Kda Regulatory Subunit Beta antibody
Anti-Pi3-Kinase Subunit P85-Beta antibody
Anti-Ptdins-3-Kinase Regulatory Subunit P85-Beta antibody
Anti-PIK3R2 antibody

Information sourced from Uniprot.org

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