| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PI3 Kinase p85 alpha (240-454) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PIK3R1 |
| Gene ID: |
5295 |
| Uniprot ID: |
P85A_HUMAN |
| Immunogen Region: |
240-454 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 240-454 of human PI3 Kinase p85 alpha (NP_852556.2). |
| Immunogen Sequence: |
EKFKREGNEKEIQRIMHNYD KLKSRISEIIDSRRRLEEDL KKQAAEYREIDKRMNSIKPD LIQLRKTRDQYLMWLTQKGV RQKKLNEWLGNENTEDQYSL VEDDEDLPHHDEKTWNVGSS NRNKAENLLRGKRDGTFLVR E |
| Tissue Specificity | Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level). |
| Post Translational Modifications | Polyubiquitinated in T-cells by CBLB.which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation. In adipose tissue, polyubiquitinated by the BCR(KBTBD2) E3 ubiquitin ligase complex.recognized by KBTBD2 through the SH2 domains, undergoes 'Lys-48'-linked polyubiquitination leading to its degradation. Phosphorylated. Tyrosine phosphorylated in response to signaling by FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylated by CSF1R. Phosphorylated by ERBB4. Phosphorylated on tyrosine residues by TEK/TIE2. Dephosphorylated by PTPRJ. Phosphorylated by PIK3CA at Ser-608.phosphorylation is stimulated by insulin and PDGF. The relevance of phosphorylation by PIK3CA is however unclear. Phosphorylated in response to KIT and KITLG/SCF. Phosphorylated by FGR. |
| Function | Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling. Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement. |
| Protein Name | Phosphatidylinositol 3-Kinase Regulatory Subunit AlphaPi3-Kinase Regulatory Subunit AlphaPi3k Regulatory Subunit AlphaPtdins-3-Kinase Regulatory Subunit AlphaPhosphatidylinositol 3-Kinase 85 Kda Regulatory Subunit AlphaPi3-Kinase Subunit P85-AlphaPtdins-3-Kinase Regulatory Subunit P85-Alpha |
| Database Links | Reactome: R-HSA-109704Reactome: R-HSA-112399Reactome: R-HSA-114604Reactome: R-HSA-1236382Reactome: R-HSA-1250342Reactome: R-HSA-1257604Reactome: R-HSA-1266695Reactome: R-HSA-1433557Reactome: R-HSA-1660499Reactome: R-HSA-180292Reactome: R-HSA-1839117Reactome: R-HSA-186763Reactome: R-HSA-1963642Reactome: R-HSA-198203Reactome: R-HSA-201556Reactome: R-HSA-202424Reactome: R-HSA-2029485Reactome: R-HSA-210993Reactome: R-HSA-2219530Reactome: R-HSA-2424491Reactome: R-HSA-2730905Reactome: R-HSA-373753Reactome: R-HSA-388841Reactome: R-HSA-389357Reactome: R-HSA-416476Reactome: R-HSA-430116Reactome: R-HSA-4420097Reactome: R-HSA-512988Reactome: R-HSA-5637810Reactome: R-HSA-5654689Reactome: R-HSA-5654695Reactome: R-HSA-5654710Reactome: R-HSA-5654720Reactome: R-HSA-5655253Reactome: R-HSA-5655291Reactome: R-HSA-5655302Reactome: R-HSA-5655332Reactome: R-HSA-5673001Reactome: R-HSA-6785807Reactome: R-HSA-6811558Reactome: R-HSA-8851907Reactome: R-HSA-8853659Reactome: R-HSA-8980692Reactome: R-HSA-9009391Reactome: R-HSA-9013026Reactome: R-HSA-9013106Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013405Reactome: R-HSA-9013408Reactome: R-HSA-9013409Reactome: R-HSA-9013420Reactome: R-HSA-9013423Reactome: R-HSA-9013424Reactome: R-HSA-9027276Reactome: R-HSA-9028335Reactome: R-HSA-9035034Reactome: R-HSA-912526Reactome: R-HSA-912631Reactome: R-HSA-9603381Reactome: R-HSA-9607240Reactome: R-HSA-9664565Reactome: R-HSA-9665348Reactome: R-HSA-9670439Reactome: R-HSA-9673767Reactome: R-HSA-9673770Reactome: R-HSA-9680350Reactome: R-HSA-9696264Reactome: R-HSA-9696270Reactome: R-HSA-9696273Reactome: R-HSA-9703465Reactome: R-HSA-9703648Reactome: R-HSA-9725370Reactome: R-HSA-983695 |
| Alternative Antibody Names | Anti-Phosphatidylinositol 3-Kinase Regulatory Subunit Alpha antibodyAnti-Pi3-Kinase Regulatory Subunit Alpha antibodyAnti-Pi3k Regulatory Subunit Alpha antibodyAnti-Ptdins-3-Kinase Regulatory Subunit Alpha antibodyAnti-Phosphatidylinositol 3-Kinase 85 Kda Regulatory Subunit Alpha antibodyAnti-Pi3-Kinase Subunit P85-Alpha antibodyAnti-Ptdins-3-Kinase Regulatory Subunit P85-Alpha antibodyAnti-PIK3R1 antibodyAnti-GRB1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
