K562 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-PIK3CA H1047R (ABT453) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: K562Predicted band size: 124kDaObserved band size: 124kDa

Anti-PIK3CA antibody (1000-1068 aa) [ABT453] (STJ197258)

Name: Anti-PIK3CA antibody (1000-1068 aa) [ABT453] (STJ197258)
SKU: STJ197258
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

ClonalityMonoclonal

HostMouse

ConjugationUnconjugated

IsotypeIgG2bk

ImmunogenSynthesized peptide derived from the human PIK3CA H1047R at the amino acid range 1000-1068

STJ197258

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General Information

Short Description	Mouse monoclonal anti-Phosphatidylinositol 4, 5-bisphosphate 3-kinase catalytic subunit alpha isoform (1000-1068 aa) for use in IHC and WB in Human samples. Datasheet included with dilution recommendations, and related reagents.
Applications	IHC/WB
Host	Mouse
Reactivity	Human
Note	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Clonality	Monoclonal
Clone ID	ABT453
Isotype	IgG2bk
Conjugation	Unconjugated
Purification	The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range	IHC-P 1:200-400 WB 500-1000
Formulation	Liquid in PBS pH7.2, 0.03% Proclin 300, with stabilizing protein.
Storage Instruction	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene Symbol	PIK3CA
Gene ID	5290
Uniprot ID	PK3CA_HUMAN
Immunogen	Synthesized peptide derived from the human PIK3CA H1047R at the amino acid range 1000-1068
Immunogen Region	1000-1068 aa
Specificity	The antibody can specifically recognize the h1047r mutation of human PIK3CA protein, and does not react with wild-type PIK3CA protein.

Additional Info

Protein Name	Phosphatidylinositol 4 -5-Bisphosphate 3-Kinase Catalytic Subunit Alpha Isoform Pi3-Kinase Subunit Alpha Pi3k-Alpha Pi3kalpha Ptdins-3-Kinase Subunit Alpha Phosphatidylinositol 4 -5-Bisphosphate 3-Kinase 110 Kda Catalytic Subunit Alpha Ptdins-3-Kinase Subunit P110-Alpha P110alpha Phosphoinositide 3-Kinase Alpha Phosphoinositide-3-Kinase Catalytic Alpha Polypeptide Serine/Threonine Protein Kinase Pik3ca
Database Links	Reactome: R-HSA-109704 Reactome: R-HSA-112399 Reactome: R-HSA-114604 Reactome: R-HSA-1236382 Reactome: R-HSA-1250342 Reactome: R-HSA-1257604 Reactome: R-HSA-1433557 Reactome: R-HSA-1660499 Reactome: R-HSA-180292 Reactome: R-HSA-1839117 Reactome: R-HSA-186763 Reactome: R-HSA-1963642 Reactome: R-HSA-198203 Reactome: R-HSA-201556 Reactome: R-HSA-202424 Reactome: R-HSA-2029485 Reactome: R-HSA-210993 Reactome: R-HSA-2219530 Reactome: R-HSA-2424491 Reactome: R-HSA-2730905 Reactome: R-HSA-373753 Reactome: R-HSA-389357 Reactome: R-HSA-416476 Reactome: R-HSA-4420097 Reactome: R-HSA-512988 Reactome: R-HSA-5637810 Reactome: R-HSA-5654689 Reactome: R-HSA-5654695 Reactome: R-HSA-5654710 Reactome: R-HSA-5654720 Reactome: R-HSA-5655253 Reactome: R-HSA-5655291 Reactome: R-HSA-5655302 Reactome: R-HSA-5655332 Reactome: R-HSA-5673001 Reactome: R-HSA-6811558 Reactome: R-HSA-8851907 Reactome: R-HSA-8853659 Reactome: R-HSA-9009391 Reactome: R-HSA-9013149 Reactome: R-HSA-9013404 Reactome: R-HSA-9027276 Reactome: R-HSA-9028335 Reactome: R-HSA-912526 Reactome: R-HSA-912631 Reactome: R-HSA-9603381 Reactome: R-HSA-9607240 Reactome: R-HSA-9664565 Reactome: R-HSA-9665348 Reactome: R-HSA-9670439 Reactome: R-HSA-9673767 Reactome: R-HSA-9673770 Reactome: R-HSA-9680350 Reactome: R-HSA-9703465 Reactome: R-HSA-9703648 Reactome: R-HSA-9725370 Reactome: R-HSA-9842640 Reactome: R-HSA-9842663 Reactome: R-HSA-9856530 Reactome: R-HSA-9927354
Cellular Localisation
Alternative Antibody Names	Anti-Phosphatidylinositol 4 -5-Bisphosphate 3-Kinase Catalytic Subunit Alpha Isoform antibody Anti-Pi3-Kinase Subunit Alpha antibody Anti-Pi3k-Alpha antibody Anti-Pi3kalpha antibody Anti-Ptdins-3-Kinase Subunit Alpha antibody Anti-Phosphatidylinositol 4 -5-Bisphosphate 3-Kinase 110 Kda Catalytic Subunit Alpha antibody Anti-Ptdins-3-Kinase Subunit P110-Alpha antibody Anti-P110alpha antibody Anti-Phosphoinositide 3-Kinase Alpha antibody Anti-Phosphoinositide-3-Kinase Catalytic Alpha Polypeptide antibody Anti-Serine/Threonine Protein Kinase Pik3ca antibody Anti-PIK3CA antibody

Information sourced from Uniprot.org

Citations

View product citations for antibody STJ197258 on CiteAb