• Western blot analysis of extracts of various cell lines, using PIGR antibody (STJ27883) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using PIGR antibody (STJ27883) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using PIGR rabbit polyclonal antibody (STJ27883) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using PIGR rabbit polyclonal antibody (STJ27883) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of A-431 cells using PIGR rabbit polyclonal antibody (STJ27883) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using PIGR rabbit polyclonal antibody (STJ27883) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using PIGR rabbit polyclonal antibody (STJ27883) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PIGR antibody (460-600) (STJ27883)

SKU:
STJ27883

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PIGR (460-600) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PIGR
Gene ID: 5284
Uniprot ID: PIGR_HUMAN
Immunogen Region: 460-600
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 460-600 of human PIGR (NP_002635.2).
Immunogen Sequence: GEPNLKVPGNVTAVLGETLK VPCHFPCKFSSYEKYWCKWN NTGCQALPSQDEGPSKAFVN CDENSRLVSLTLNLVTRADE GWYWCGVKQGHFYGETAAVY VAVEERKAAGSRDVSLAKAD AAPDEKVLDSGFREIENKAI Q
Post Translational Modifications N-glycosylated. N-glycosylation is required for anchoring IgA molecules to mucus, but is not necessary for Ig binding.
Function Polymeric immunoglobulin receptor: Mediates selective transcytosis of polymeric IgA and IgM across mucosal epithelial cells. Binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process, a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment. Secretory component: Through its N-linked glycans ensures anchoring of secretory IgA (sIgA) molecules to mucus lining the epithelial surface to neutralize extracellular pathogens. On its own (free form) may act as a non-specific microbial scavenger to prevent pathogen interaction with epithelial cells.
Protein Name Polymeric Immunoglobulin Receptor
Pigr
Poly-Ig Receptor
Hepatocellular Carcinoma-Associated Protein Tb6 Cleaved Into - Secretory Component
Database Links Reactome: R-HSA-6798695
Cellular Localisation Polymeric Immunoglobulin Receptor: Cell Membrane
Single-Pass Type I Membrane Protein
Secretory Component: Secreted
Alternative Antibody Names Anti-Polymeric Immunoglobulin Receptor antibody
Anti-Pigr antibody
Anti-Poly-Ig Receptor antibody
Anti-Hepatocellular Carcinoma-Associated Protein Tb6 Cleaved Into - Secretory Component antibody
Anti-PIGR antibody

Information sourced from Uniprot.org

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