Anti-PIAS2 antibody (Internal) (STJ70889)

SKU:
STJ70889-100

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Host: Goat
Applications: Pep-ELISA/IHC
Reactivity: Human/Mouse/Rat/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-PIAS2 (Internal) is suitable for use in ELISA and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-Recommended concentration, 3-5µg/ml
ELISA-antibody detection limit dilution 1:8000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: PIAS2
Gene ID: 9063
Uniprot ID: PIAS2_HUMAN
Immunogen Region: Internal
Accession Number: NP_775298.1; NP_004662.2
Specificity: This antibody is expected to recognise both reported isoforms (NP_775298.1 and NP_004662.2). No cross-reactivity expected with PIAS1, 3 and 4.
Immunogen Sequence: KEAMKVSSQPCTKIE
Post Translational Modifications Sumoylated.
Function Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys-160'.
Protein Name E3 Sumo-Protein Ligase Pias2
Androgen Receptor-Interacting Protein 3
Arip3
Dab2-Interacting Protein
Dip
E3 Sumo-Protein Transferase Pias2
Msx-Interacting Zinc Finger Protein
Miz1
Pias-Ny Protein
Protein Inhibitor Of Activated Stat X
Protein Inhibitor Of Activated Stat2
Database Links Reactome: R-HSA-3108214 O75928-1
Reactome: R-HSA-3232118 O75928-1
Reactome: R-HSA-3232142 O75928-1
Reactome: R-HSA-3899300 O75928-1
Reactome: R-HSA-4090294
Reactome: R-HSA-4551638 O75928-2
Reactome: R-HSA-5617472
Cellular Localisation Nucleus Speckle
Nucleus
Pml Body
Colocalizes At Least Partially With Promyelocytic Leukemia Nuclear Bodies (Pml Nbs)
Colocalizes With Sumo1 In Nuclear Granules
Alternative Antibody Names Anti-E3 Sumo-Protein Ligase Pias2 antibody
Anti-Androgen Receptor-Interacting Protein 3 antibody
Anti-Arip3 antibody
Anti-Dab2-Interacting Protein antibody
Anti-Dip antibody
Anti-E3 Sumo-Protein Transferase Pias2 antibody
Anti-Msx-Interacting Zinc Finger Protein antibody
Anti-Miz1 antibody
Anti-Pias-Ny Protein antibody
Anti-Protein Inhibitor Of Activated Stat X antibody
Anti-Protein Inhibitor Of Activated Stat2 antibody
Anti-PIAS2 antibody
Anti-PIASX antibody

Information sourced from Uniprot.org

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