• Immunofluorescence analysis of A-549 cells using PHYHD1 antibody (STJ29288). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using PHYHD1 antibody (STJ29288) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-PHYHD1 antibody (1-291) (STJ29288)

SKU:
STJ29288

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PHYHD1 (1-291) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PHYHD1
Gene ID: 254295
Uniprot ID: PHYD1_HUMAN
Immunogen Region: 1-291
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-291 of human PHYHD1 (NP_001094346.1).
Immunogen Sequence: MACLSPSQLQKFQQDGFLVL EGFLSAEECVAMQQRIGEIV AEMDVPLHCRTEFSTQEEEQ LRAQGSTDYFLSSGDKIRFF FEKGVFDEKGNFLVPPEKSI NKIGHALHAHDPVFKSITHS FKVQTLARSLGLQMPVVVQS MYIFKQPHFGGEVSPHQDAS FLYTEPLGRVLGVWIAVEDA TLENGCLWFIPGSHTSGVSR RMVRAPVGSAPGTSFLGSEP ARDNSLFVPTPVQRGALVL
Function 2-oxoglutarate(2OG)-dependent dioxygenase that catalyzes the conversion of 2-oxoglutarate to succinate and CO(2) in an iron-dependent manner. However, does not couple 2OG turnover to the hydroxylation of acyl-coenzyme A derivatives, implying that it is not directly involved in phytanoyl coenzyme-A metabolism. Does not show detectable activity towards fatty acid CoA thioesters. Isoform 2: Isoform 2 probably lacks enzyme activity. Isoform 3: Isoform 3 probably lacks enzyme activity.
Protein Name Phytanoyl-Coa Dioxygenase Domain-Containing Protein 1
Protein Phyhd1
Alternative Antibody Names Anti-Phytanoyl-Coa Dioxygenase Domain-Containing Protein 1 antibody
Anti-Protein Phyhd1 antibody
Anti-PHYHD1 antibody

Information sourced from Uniprot.org

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