Anti-Phospho-XRCC5-Thr714 antibody (683-732 aa) (STJ91033)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Ku80 around the phosphorylation site of Thr714 at the amino acid range 683-732
General Information
| Short Description | Rabbit polyclonal anti-Phospho-X-ray repair cross-complementing protein 5-Thr714 (683-732 aa) for use in WB, IHC, IF and ELISA in Human and Monkey samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Monkey |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:10000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | XRCC5 |
| Gene ID | 7520 |
| Uniprot ID | XRCC5_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human Ku80 around the phosphorylation site of Thr714 at the amino acid range 683-732 |
| Immunogen Region | 683-732 aa |
| Specificity | Phospho-Ku-80 (T714) Polyclonal Antibody detects endogenous levels of Ku-80 protein only when phosphorylated at T714. |
Additional Info
| Function | Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA. Required for double-strand break repair and V(D)J recombination. Also has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. During NHEJ, the XRCC5-XRRC6 dimer performs the recognition step: it recognizes and binds to the broken ends of the DNA and protects them from further resection. Binding to DNA may be mediated by XRCC6. The XRCC5-XRRC6 dimer acts as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5-XRRC6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. The XRCC5-XRRC6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5-XRRC6 dimer together with APEX1 acts as a negative regulator of transcription. In association with NAA15, the XRCC5-XRRC6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. As part of the DNA-PK complex, involved in the early steps of ribosome assembly by promoting the processing of precursor rRNA into mature 18S rRNA in the small-subunit processome. Binding to U3 small nucleolar RNA, recruits PRKDC and XRCC5/Ku86 to the small-subunit processome. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway. |
| Protein Name | X-Ray Repair Cross-Complementing Protein 586 Kda Subunit Of Ku AntigenAtp-Dependent Dna Helicase 2 Subunit 2Atp-Dependent Dna Helicase Ii 80 Kda SubunitCtc Box-Binding Factor 85 Kda SubunitCtc85CtcbfDna Repair Protein Xrcc5Ku80Ku86Lupus Ku Autoantigen Protein P86Nuclear Factor IvThyroid-Lupus AutoantigenTlaaX-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 5Double-Strand-Break Rejoining |
| Database Links | Reactome: R-HSA-164843Reactome: R-HSA-1834949Reactome: R-HSA-3270619Reactome: R-HSA-5693571Reactome: R-HSA-6798695 |
| Cellular Localisation | NucleusNucleolusChromosome |
| Alternative Antibody Names | Anti-X-Ray Repair Cross-Complementing Protein 5 antibodyAnti-86 Kda Subunit Of Ku Antigen antibodyAnti-Atp-Dependent Dna Helicase 2 Subunit 2 antibodyAnti-Atp-Dependent Dna Helicase Ii 80 Kda Subunit antibodyAnti-Ctc Box-Binding Factor 85 Kda Subunit antibodyAnti-Ctc85 antibodyAnti-Ctcbf antibodyAnti-Dna Repair Protein Xrcc5 antibodyAnti-Ku80 antibodyAnti-Ku86 antibodyAnti-Lupus Ku Autoantigen Protein P86 antibodyAnti-Nuclear Factor Iv antibodyAnti-Thyroid-Lupus Autoantigen antibodyAnti-Tlaa antibodyAnti-X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 5 antibodyAnti-Double-Strand-Break Rejoining antibodyAnti-XRCC5 antibodyAnti-G22P2 antibody |
Information sourced from Uniprot.org