Anti-Phospho-WAVE2-Tyr-150 antibody (STJA0041450)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenPhospho-WAVE (Tyr-150) peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-150 in human WAVE2. This sequence has high homology with similar regions in rat and mouse WAVE2, and has homology to the conserved sites in WAVE1 (Ty
General Information
| Short Description | Rabbit polyclonal anti-Phospho-WAVE2-Tyr-150 for use in ELISA, WB and ICC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB/ICC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Antigen Affinity Purified |
| Dilution Range | WB 1:1000ICC 1:200 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Storage at-20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20°C. |
Target Information
| Gene Symbol | WASF2 |
| Gene ID | 10163 |
| Uniprot ID | WASF2_HUMAN |
| Immunogen | Phospho-WAVE (Tyr-150) peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-150 in human WAVE2. This sequence has high homology with similar regions in rat and mouse WAVE2, and has homology to the conserved sites in WAVE1 (Ty |
| Immunogen Sequence | Human |
| Specificity | This antibody was cross-adsorbed to phospho-WAVE (Tyr-125) and unphosphorylated WAVE (Tyr-150) peptides before affinity purification using phospho-WAVE (Tyr-150) peptide. In western blots, the antibody detects a 78 kDa* corresponding to phosphorylate |
Additional Info
| Tissue Specificity | Expressed in all tissues with strongest expression in placenta, lung, and peripheral blood leukocytes, but not in skeletal muscle. |
| Function | Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. |
| Protein Name | Actin-Binding Protein Wasf2Protein Wave-2Verprolin Homology Domain-Containing Protein 2Wiskott-Aldrich Syndrome Protein Family Member 2Wasp Family Protein Member 2 |
| Database Links | Reactome: R-HSA-2029482Reactome: R-HSA-4420097Reactome: R-HSA-5663213Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013423Reactome: R-HSA-9664422 |
| Cellular Localisation | CytoplasmCytoskeletonCell ProjectionLamellipodiumBasolateral Cell MembraneAt The Interface Between The Lamellipodial Actin Meshwork And The Membrane |
| Alternative Antibody Names | Anti-Actin-Binding Protein Wasf2 antibodyAnti-Protein Wave-2 antibodyAnti-Verprolin Homology Domain-Containing Protein 2 antibodyAnti-Wiskott-Aldrich Syndrome Protein Family Member 2 antibodyAnti-Wasp Family Protein Member 2 antibodyAnti-WASF2 antibodyAnti-WAVE2 antibody |
Information sourced from Uniprot.org