• Western blot analysis of JK cells using Phospho-Vav3 (Y173) Polyclonal Antibody

Anti-Phospho-VAV3-Tyr173 antibody (STJ90999)

SKU:
STJ90999

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Guanine nucleotide exchange factor VAV3-Tyr173 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VAV3
Gene ID: 10451
Uniprot ID: VAV3_HUMAN
Specificity: Phospho-Vav3 (Y173) Polyclonal Antibody detects endogenous levels of Vav3 protein only when phosphorylated at Y173.
Immunogen: Synthesized phospho-peptide around the phosphorylation site of human Vav3 (phospho Tyr173)
Post Translational Modifications Phosphorylated. Phosphorylation can be mediated by ROS1. In osteoclasts, undergoes tyrosine phosphorylation in response to CSF1.
Function Exchange factor for GTP-binding proteins RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis. Its recruitment by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. May be important for integrin-mediated signaling, at least in some cell types. In osteoclasts, along with SYK tyrosine kinase, required for signaling through integrin alpha-v/beta-1 (ITAGV-ITGB1), a crucial event for osteoclast proper cytoskeleton organization and function. This signaling pathway involves RAC1, but not RHO, activation. Necessary for proper wound healing. In the course of wound healing, required for the phagocytotic cup formation preceding macrophage phagocytosis of apoptotic neutrophils. Responsible for integrin beta-2 (ITGB2)-mediated macrophage adhesion and, to a lesser extent, contributes to beta-3 (ITGB3)-mediated adhesion. Does not affect integrin beta-1 (ITGB1)-mediated adhesion.
Protein Name Guanine Nucleotide Exchange Factor Vav3
Vav-3
Database Links Reactome: R-HSA-114604
Reactome: R-HSA-193648
Reactome: R-HSA-2029482
Reactome: R-HSA-2424491
Reactome: R-HSA-2871796
Reactome: R-HSA-2871809
Reactome: R-HSA-3928665
Reactome: R-HSA-416482
Reactome: R-HSA-4420097
Reactome: R-HSA-5218920
Reactome: R-HSA-8980692
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013408
Reactome: R-HSA-9664422
Reactome: R-HSA-9748787
Alternative Antibody Names Anti-Guanine Nucleotide Exchange Factor Vav3 antibody
Anti-Vav-3 antibody
Anti-VAV3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance