• Western blot analysis of extracts of HeLa cells, using Phospho-VANGL2-S79/S82/S84 antibody (STJ11103744) at 1:1000 dilution. HeLa cells were treated by wnt (100ng/ml) for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of extracts of various cell lines, using Phospho-VANGL2-S79/S82/S84 Rabbit monoclonal antibody antibody (STJ11103744) at1:1000 dilution. HeLa and C6 cells were treated by wnt (100ng/ml) for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-VANGL2-S79/S82/S84 antibody [S4MR] (STJ11103744)

SKU:
STJ11103744

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Phospho-VANGL2-S79/S82/S84 is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VANGL2
Gene ID: 57216
Uniprot ID: VANG2_HUMAN
Immunogen: A phospho specific peptide corresponding to residues surrounding S79/S82/S84 of human VANGL2
Immunogen Sequence: RDDNWGETTTVVTGTSEHSI SHDDLTRIAKDMEDSVPLDC
Function Involved in the control of early morphogenesis and patterning of both axial midline structures and the development of neural plate. Plays a role in the regulation of planar cell polarity, particularly in the orientation of stereociliary bundles in the cochlea. Required for polarization and movement of myocardializing cells in the outflow tract and seems to act via RHOA signaling to regulate this process. Required for cell surface localization of FZD3 and FZD6 in the inner ear.
Protein Name Vang-Like Protein 2
Loop-Tail Protein 1 Homolog
Strabismus 1
Van Gogh-Like Protein 2
Database Links Reactome: R-HSA-4086400
Reactome: R-HSA-4608870
Reactome: R-HSA-9696264
Reactome: R-HSA-9696270
Reactome: R-HSA-9696273
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Vang-Like Protein 2 antibody
Anti-Loop-Tail Protein 1 Homolog antibody
Anti-Strabismus 1 antibody
Anti-Van Gogh-Like Protein 2 antibody
Anti-VANGL2 antibody
Anti-KIAA1215 antibody
Anti-STB1 antibody

Information sourced from Uniprot.org

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