• Western blot analysis of extracts of 293T cells, using Phospho-ULK1-S555 antibody (STJ116381) at 1:1000 dilution. 293T cells were treated by Oligomycin (0. 5 uM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of 293T cells, using Phospho-ULK1-S555 antibody (STJ116381) at 1:1000 dilution. 293T cells were treated by Oligomycin (0. 5 uM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-ULk1-S555 antibody (STJ116381)

SKU:
STJ116381

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-ULk1-S555 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ULK1
Gene ID: 8408
Uniprot ID: ULK1_HUMAN
Immunogen: A synthetic phosphorylated peptide around S555 of human Phospho-ULK1-S555 (NP_003556.1).
Immunogen Sequence: RLHSA
Tissue Specificity Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
Post Translational Modifications Autophosphorylated. Phosphorylated under nutrient-rich conditions.dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1. In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy. Ubiquitinated via 'Lys-63'-linkage by a complex composed of AMBRA1 and TRAF6 following autophagy induction, promoting ULK1 stability and kinase activity. Deubiquitinated by USP20.leading to ULK1 stability and autophagy initiation. Acetylated by KAT5/TIP60 under autophagy induction, promoting protein kinase activity.
Function Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR.however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy. Phosphorylates FLCN, promoting autophagy. Phosphorylates AMBRA1 in response to autophagy induction, releasing AMBRA1 from the cytoskeletal docking site to induce autophagosome nucleation. Phosphorylates ATG4B, leading to inhibit autophagy by decreasing both proteolytic activation and delipidation activities of ATG4B.
Protein Name Serine/Threonine-Protein Kinase Ulk1
Autophagy-Related Protein 1 Homolog
Atg1
Hatg1
Unc-51-Like Kinase 1
Database Links Reactome: R-HSA-1632852
Reactome: R-HSA-5357905
Reactome: R-HSA-8854214
Reactome: R-HSA-8876198
Reactome: R-HSA-8934903
Cellular Localisation Cytoplasm
Cytosol
Preautophagosomal Structure
Under Starvation Conditions
Is Localized To Puncate Structures Primarily Representing The Isolation Membrane That Sequesters A Portion Of The Cytoplasm Resulting In The Formation Of An Autophagosome
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Ulk1 antibody
Anti-Autophagy-Related Protein 1 Homolog antibody
Anti-Atg1 antibody
Anti-Hatg1 antibody
Anti-Unc-51-Like Kinase 1 antibody
Anti-ULK1 antibody
Anti-KIAA0722 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance