• Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Tyk2-Y1054/1055 rabbit polyclonal antibody (STJ113493) at 1:1000 dilution. NIH/3T3 cells were treated by IFN-Alpha (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Tyk2-Y1054/1055 Rabbit polyclonal antibody (STJ113493) at 1:1000 dilution. NIH/3T3 cells were treated by IFN-Alpha (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Phospho-Tyk2-Y1054/1055 antibody (STJ113493)

SKU:
STJ113493

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Tyk2-Y1054/1055 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TYK2
Gene ID: 7297
Uniprot ID: TYK2_HUMAN
Immunogen: A synthetic phosphorylated peptide around Y1054 & Y1055 of human Tyk2 (NP_003322.3).
Immunogen Sequence: HEYYRV
Tissue Specificity Observed in all cell lines analyzed. Expressed in a variety of lymphoid and non-lymphoid cell lines.
Post Translational Modifications Phosphorylated. Phosphorylation by JAK1 at Tyr-1054 and Tyr-1055 induces kinase activation.
Function Tyrosine kinase of the non-receptor type involved in numerous cytokines and interferons signaling, which regulates cell growth, development, cell migration, innate and adaptive immunity. Plays both structural and catalytic roles in numerous interleukins and interferons (IFN-alpha/beta) signaling. Associates with heterodimeric cytokine receptor complexes and activates STAT family members including STAT1, STAT3, STAT4 or STAT6. The heterodimeric cytokine receptor complexes are composed of (1) a TYK2-associated receptor chain (IFNAR1, IL12RB1, IL10RB or IL13RA1), and (2) a second receptor chain associated either with JAK1 or JAK2. In response to cytokine-binding to receptors, phosphorylates and activates receptors (IFNAR1, IL12RB1, IL10RB or IL13RA1), creating docking sites for STAT members. In turn, recruited STATs are phosphorylated by TYK2 (or JAK1/JAK2 on the second receptor chain), form homo- and heterodimers, translocate to the nucleus, and regulate cytokine/growth factor responsive genes. Negatively regulates STAT3 activity by promototing phosphorylation at a specific tyrosine that differs from the site used for signaling.
Protein Name Non-Receptor Tyrosine-Protein Kinase Tyk2
Database Links Reactome: R-HSA-1059683
Reactome: R-HSA-110056
Reactome: R-HSA-112411
Reactome: R-HSA-449836
Reactome: R-HSA-6783783
Reactome: R-HSA-6785807
Reactome: R-HSA-6788467
Reactome: R-HSA-8854691
Reactome: R-HSA-8984722
Reactome: R-HSA-9020591
Reactome: R-HSA-9020933
Reactome: R-HSA-9020956
Reactome: R-HSA-909733
Reactome: R-HSA-912694
Reactome: R-HSA-9674555
Reactome: R-HSA-9679191
Reactome: R-HSA-9705462
Reactome: R-HSA-9705671
Alternative Antibody Names Anti-Non-Receptor Tyrosine-Protein Kinase Tyk2 antibody
Anti-TYK2 antibody

Information sourced from Uniprot.org

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