Anti-Phospho-TSC2-Ser664 antibody (STJ196461)

SKU:
STJ196461

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Tuberin-Ser664 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000-2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TSC2
Gene ID: 7249
Uniprot ID: TSC2_HUMAN
Specificity: This antibody detects endogenous levels of Human Tuberin/TSC2 (phospho-Ser664)
Immunogen: Synthesized phosho peptide around human Tuberin (Ser664)
Post Translational Modifications Phosphorylation at Ser-939 and Thr-1462 by PKB/AKT1 in response to insulin signaling and growth factor stimulation inhibits the ability of the TSC-TBC complex to suppress mTORC1 signaling: phosphorylation promotes dissociation of the TSC-TBC complex from lysosomal membranes, leading to activation of mTORC1 by RHEB. Phosphorylation at Ser-1387, Ser-1418 or Ser-1420 does not affect interaction with TSC1. Phosphorylation by AMPK activates it and leads to negative regulation of the mTORC1 complex. Phosphorylated at Ser-1798 by RPS6KA1.phosphorylation inhibits TSC2 ability to suppress mTORC1 signaling. Phosphorylated by DAPK1. Ubiquitinated by the DCX(FBXW5) E3 ubiquitin-protein ligase complex, leading to its subsequent degradation. Ubiquitinated by MYCBP2 independently of its phosphorylation status leading to subsequent degradation.association with TSC1 protects from ubiquitination.
Function Catalytic component of the TSC-TBC complex, a multiprotein complex that acts as a negative regulator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. Within the TSC-TBC complex, TSC2 acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. In absence of nutrients, the TSC-TBC complex inhibits mTORC1, thereby preventing phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) by the mTORC1 signaling. The TSC-TBC complex is inactivated in response to nutrients, relieving inhibition of mTORC1. Involved in microtubule-mediated protein transport via its ability to regulate mTORC1 signaling. Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5.
Protein Name Tuberin
Tuberous Sclerosis 2 Protein
Database Links Reactome: R-HSA-1632852
Reactome: R-HSA-165181
Reactome: R-HSA-198323
Reactome: R-HSA-380972
Reactome: R-HSA-5628897
Reactome: R-HSA-5674400
Reactome: R-HSA-8854214
Cellular Localisation Lysosome Membrane
Peripheral Membrane Protein
Cytoplasm
Cytosol
Recruited To Lysosomal Membranes In A Rheb-Dependent Process In Absence Of Nutrients
In Response To Insulin Signaling And Phosphorylation By Pkb/Akt1
The Complex Dissociates From Lysosomal Membranes And Relocalizes To The Cytosol
Alternative Antibody Names Anti-Tuberin antibody
Anti-Tuberous Sclerosis 2 Protein antibody
Anti-TSC2 antibody
Anti-TSC4 antibody

Information sourced from Uniprot.org

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