Anti-Phospho-TSC2-Ser1254 antibody (STJ196459)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized phosho peptide around human Tuberin (Ser1254)
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Tuberin-Ser1254 for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Dilution Range | WB 1:1000-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TSC2 |
| Gene ID | 7249 |
| Uniprot ID | TSC2_HUMAN |
| Immunogen | Synthesized phosho peptide around human Tuberin (Ser1254) |
| Specificity | This antibody detects endogenous levels of Human Mouse Rat Tuberin/TSC2 (phospho-Ser1254) |
Additional Info
| Post Translational Modifications | Phosphorylation at Ser-939 and Thr-1462 by PKB/AKT1 in response to insulin signaling and growth factor stimulation inhibits the ability of the TSC-TBC complex to suppress mTORC1 signaling: phosphorylation promotes dissociation of the TSC-TBC complex from lysosomal membranes, leading to activation of mTORC1 by RHEB. Phosphorylation at Ser-1387, Ser-1418 or Ser-1420 does not affect interaction with TSC1. Phosphorylation by AMPK activates it and leads to negative regulation of the mTORC1 complex. Phosphorylated at Ser-1798 by RPS6KA1.phosphorylation inhibits TSC2 ability to suppress mTORC1 signaling. Phosphorylated by DAPK1. Ubiquitinated by the DCX(FBXW5) E3 ubiquitin-protein ligase complex, leading to its subsequent degradation. Ubiquitinated by MYCBP2 independently of its phosphorylation status leading to subsequent degradation.association with TSC1 protects from ubiquitination. |
| Function | Catalytic component of the TSC-TBC complex, a multiprotein complex that acts as a negative regulator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. Within the TSC-TBC complex, TSC2 acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. In absence of nutrients, the TSC-TBC complex inhibits mTORC1, thereby preventing phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) by the mTORC1 signaling. The TSC-TBC complex is inactivated in response to nutrients, relieving inhibition of mTORC1. Involved in microtubule-mediated protein transport via its ability to regulate mTORC1 signaling. Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5. |
| Protein Name | TuberinTuberous Sclerosis 2 Protein |
| Database Links | Reactome: R-HSA-1632852Reactome: R-HSA-165181Reactome: R-HSA-198323Reactome: R-HSA-380972Reactome: R-HSA-5628897Reactome: R-HSA-5674400Reactome: R-HSA-8854214 |
| Cellular Localisation | Lysosome MembranePeripheral Membrane ProteinCytoplasmCytosolRecruited To Lysosomal Membranes In A Rheb-Dependent Process In Absence Of NutrientsIn Response To Insulin Signaling And Phosphorylation By Pkb/Akt1The Complex Dissociates From Lysosomal Membranes And Relocalizes To The Cytosol |
| Alternative Antibody Names | Anti-Tuberin antibodyAnti-Tuberous Sclerosis 2 Protein antibodyAnti-TSC2 antibodyAnti-TSC4 antibody |
Information sourced from Uniprot.org