Anti-Phospho-TNNI3-Ser43 antibody (STJA0003810)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic phospho-peptide corresponding to amino acid residues surrounding Ser43 conjugated to KLH.
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Cardiac Troponin I-Ser43 for use in WB in Human, Mouse, Rat, Bovine, Canine, Non-Human Primates, Sheep, Xenopus and Zebrafish samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat/Bovine/Canine/Non-Human Primates/Sheep/Xenopus/Zebrafish |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | This antibody was antigen affinity purified from pooled serum. |
| Dilution Range | WB 1:1000 |
| Formulation | 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Tnni3 |
| Gene ID | 21954 |
| Uniprot ID | TNNI3_MOUSE |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser43 conjugated to KLH. |
Additional Info
| Post Translational Modifications | Phosphorylated at Ser-23 and Ser-24 by PRKD1.phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-32. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). Phosphorylated at Ser-43 and Ser-45 by PRKCE.phosphorylation increases myocardium contractile dysfunction. |
| Function | Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. |
| Protein Name | Troponin I - Cardiac MuscleCardiac Troponin I |
| Database Links | Reactome: R-MMU-390522Reactome: -MMU-5578775 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Troponin I - Cardiac Muscle antibodyAnti-Cardiac Troponin I antibodyAnti-Tnni3 antibody |
Information sourced from Uniprot.org