Anti-Phospho-TLN1-Ser-425 antibody (STJA0005613)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenPhospho-Talin (Ser-425) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-425 in human Talin 1. This sequence is conserved in rat, mouse, and chicken Talin 1, as well as in Talin 2 (Ser-428).
General Information
| Short Description | Rabbit polyclonal antibody anti-Phospho-Talin-Ser-425 is suitable for use in Western Blot and Immunocytochemistry research applications. |
| Applications | WB/ICC |
| Host | Rabbit |
| Reactivity | Chicken/Human/Mouse/Rat/Xenopus |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Antigen Affinity Purified |
| Dilution Range | WB 1:1000ICC 1:200 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TLN1 |
| Gene ID | 7094 |
| Uniprot ID | TLN1_HUMAN |
| Immunogen | Phospho-Talin (Ser-425) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-425 in human Talin 1. This sequence is conserved in rat, mouse, and chicken Talin 1, as well as in Talin 2 (Ser-428). |
| Specificity | The antibody detects a 240 kDa* protein corresponding to the molecular mass of Talin on SDS-PAGE immunoblots of human A431, rat PC12, and rabbit fibroblast cells treated with Calyculin A, but is not observed after lambda phosphatase treatment. |
Additional Info
| Function | High molecular weight cytoskeletal protein concentrated at regions of cell-matrix and cell-cell contacts. Involved in connections of major cytoskeletal structures to the plasma membrane. With KANK1 co-organize the assembly of cortical microtubule stabilizing complexes (CMSCs) positioned to control microtubule-actin crosstalk at focal adhesions (FAs) rims. |
| Protein Name | Talin-1 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-354192Reactome: R-HSA-354194Reactome: R-HSA-372708Reactome: R-HSA-381038Reactome: R-HSA-399955Reactome: R-HSA-445355Reactome: R-HSA-5674135Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-9649948Reactome: R-HSA-9656223 |
| Cellular Localisation | Cell ProjectionRuffle MembranePeripheral Membrane ProteinCytoplasmic SideCytoplasmCytoskeletonCell SurfaceCell JunctionFocal AdhesionColocalizes With Layn At The Membrane RufflesLocalized Preferentially In Focal Adhesions Than Fibrillar Adhesions |
| Alternative Antibody Names | Anti-Talin-1 antibodyAnti-TLN1 antibodyAnti-KIAA1027 antibodyAnti-TLN antibody |
Information sourced from Uniprot.org