Anti-Phospho-TIFA-T9-T9 antibody [SRM15132] (STJ11109862)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide. This information is considered to be commercially sensitive.
General Information
| Short Description | Rabbit monoclonal Phospho-TIFA-T9-T9 antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM15132 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB, 1:2000-1:6000ELISA, Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TIFA |
| Gene ID | 92610 |
| Uniprot ID | TIFA_HUMAN |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
Additional Info
| Post Translational Modifications | Phosphorylated at Thr-9 following detection of ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose) by ALPK1. Phosphorylation at Thr-9 by ALPK1 leads to the formation of an intermolecular binding between the FHA domain and phosphorylated Thr-9, promoting TIFA oligomerization and TIFA-mediated NF-kappa-B activation. |
| Function | Adapter molecule that plays a key role in the activation of pro-inflammatory NF-kappa-B signaling following detection of bacterial pathogen-associated molecular pattern metabolites (PAMPs). Promotes activation of an innate immune response by inducing the oligomerization and polyubiquitination of TRAF6, which leads to the activation of TAK1 and IKK through a proteasome-independent mechanism. TIFA-dependent innate immune response is triggered by ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose), a potent PAMP present in all Gram-negative and some Gram-positive bacteria: ADP-Heptose is recognized by ALPK1, which phosphorylates TIFA at Thr-9, leading to TIFA homooligomerization and subsequent activation of pro-inflammatory NF-kappa-B signaling. |
| Protein Name | Traf-Interacting Protein With Fha Domain-Containing Protein APutative Mapk-Activating Protein Pm14Putative Nf-Kappa-B-Activating Protein 20Traf2-Binding Protein |
| Database Links | Reactome: R-HSA-445989Reactome: R-HSA-9645460 |
| Cellular Localisation | CytoplasmColocalizes With Lysosomal Marker Lamp2 Following Homooligomerization And Subsequent Activation |
| Alternative Antibody Names | Anti-Traf-Interacting Protein With Fha Domain-Containing Protein A antibodyAnti-Putative Mapk-Activating Protein Pm14 antibodyAnti-Putative Nf-Kappa-B-Activating Protein 20 antibodyAnti-Traf2-Binding Protein antibodyAnti-TIFA antibodyAnti-T2BP antibody |
Information sourced from Uniprot.org