• Western blot analysis of extracts of PC-12 cells, using Phospho-TH-S40 polyclonal antibody (STJ11101143) at 1:1000 dilution or TH antibody (A0028). PC-12 cells were treated by Forskolin (30 uM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3s.
  • Western blot analysis of lysates from PC-12 cells, using Phospho-Tyrosine Hydroxylase-S40 polyclonal antibody (STJ11101143) at 1:1000 dilution or Tyrosine Hydroxylase antibody (A0028). PC-12 cells were treated by Forskolin (30 uM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-Phospho-TH-S40 antibody (STJ11101143)

SKU:
STJ11101143

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Host: Rabbit
Applications: WB
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-TH-S40 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TH
Gene ID: 7054
Uniprot ID: TY3H_HUMAN
Immunogen: A synthetic phosphorylated peptide around S40 of human Tyrosine Hydroxylase (NP_000351.2?).
Immunogen Sequence: RQSLI
Tissue Specificity Mainly expressed in the brain and adrenal glands.
Post Translational Modifications Phosphorylated on Ser-19, Ser-62 and Ser-71 by several protein kinases with different site specificities. Phosphorylation at Ser-62 and Ser-71 leads to an increase of TH activity. Phosphorylation at Ser-71 activates the enzyme and also counteracts the feedback inhibition of TH by catecholamines. Phosphorylation of Ser-19 and Ser-62 triggers the proteasomal degradation of TH through the ubiquitin-proteasome pathway. Phosphorylation at Ser-62 facilitates transport of TH from the soma to the nerve terminals via the microtubule network. Phosphorylation at Ser-19 induces the high-affinity binding to the 14-3-3 protein YWHAG.this interaction may influence the phosphorylation and dephosphorylation of other sites. Ser-19 increases the phosphorylation at Ser-71 in a hierarchical manner, leading to increased activity.
Function Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of cathecolamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa. In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity. Positively regulates the regression of retinal hyaloid vessels during postnatal development. Isoform 5: Lacks catalytic activity. Isoform 6: Lacks catalytic activity.
Protein Name Tyrosine 3-Monooxygenase
Tyrosine 3-Hydroxylase
Th
Database Links Reactome: R-HSA-209905
Cellular Localisation Cytoplasm
Perinuclear Region
Nucleus
Cell Projection
Axon
Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle
When Phosphorylated At Ser-19 Shows A Nuclear Distribution And When Phosphorylated At Ser-31 As Well At Ser-40 Shows A Cytosolic Distribution
Expressed In Dopaminergic Axons And Axon Terminals
Alternative Antibody Names Anti-Tyrosine 3-Monooxygenase antibody
Anti-Tyrosine 3-Hydroxylase antibody
Anti-Th antibody
Anti-TH antibody
Anti-TYH antibody

Information sourced from Uniprot.org

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