Anti-Phospho-TERF1-Ser219 antibody (185-234 aa) (STJ90560)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Telomeric Repeat Binding Factor 1 around the phosphorylation site of Ser219 at the amino acid range 185-234
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Telomeric repeat-binding factor 1-Ser219 (185-234 aa) for use in WB and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:5000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TERF1 |
| Gene ID | 7013 |
| Uniprot ID | TERF1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human Telomeric Repeat Binding Factor 1 around the phosphorylation site of Ser219 at the amino acid range 185-234 |
| Immunogen Region | 185-234 aa |
| Specificity | Phospho-TRF1 (S219) Polyclonal Antibody detects endogenous levels of TRF1 protein only when phosphorylated at S219. |
Additional Info
| Post Translational Modifications | Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage. ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA. Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome. |
| Function | Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends.without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. |
| Protein Name | Telomeric Repeat-Binding Factor 1Nima-Interacting Protein 2Ttaggg Repeat-Binding Factor 1Telomeric Protein Pin2/Trf1 |
| Database Links | Reactome: R-HSA-110328Reactome: R-HSA-110329Reactome: R-HSA-110330Reactome: R-HSA-110331Reactome: R-HSA-1221632Reactome: R-HSA-171306Reactome: R-HSA-171319Reactome: R-HSA-174411Reactome: R-HSA-174414Reactome: R-HSA-174417Reactome: R-HSA-174430Reactome: R-HSA-174437Reactome: R-HSA-2559586Reactome: R-HSA-9670095 |
| Cellular Localisation | NucleusCytoplasmCytoskeletonSpindleChromosomeTelomereColocalizes With Telomeric Dna In Interphase And Prophase CellsTelomeric Localization Decreases In MetaphaseAnaphase And TelophaseAssociates With The Mitotic SpindleColocalizes With Triobp Isoform 1 At The Telomeres In Interphase |
| Alternative Antibody Names | Anti-Telomeric Repeat-Binding Factor 1 antibodyAnti-Nima-Interacting Protein 2 antibodyAnti-Ttaggg Repeat-Binding Factor 1 antibodyAnti-Telomeric Protein Pin2/Trf1 antibodyAnti-TERF1 antibodyAnti-PIN2 antibodyAnti-TRBF1 antibodyAnti-TRF antibodyAnti-TRF1 antibody |
Information sourced from Uniprot.org