• Western blot analysis of extracts of 293T cells, using Phospho-TBC1D4-T642 antibody (STJ117889) at 1:2000 dilution. 293T cells were treated by IGF-1 (50ng/mL) for 5 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 30s.
  • Western blot analysis of lysates from 293T cells, using Phospho-TBC1D4-T642 Rabbit polyclonal antibody (STJ117889) at 1:2000 dilution. 293T cells were treated by IGF-1 (50ng/mL) for 5 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-Phospho-TBC1D4-T642 antibody (STJ117889)

SKU:
STJ117889

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-TBC1D4-T642 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TBC1D4
Gene ID: 9882
Uniprot ID: TBCD4_HUMAN
Immunogen: A synthetic phosphorylated peptide around T642 of human TBC1D4 (NP_055647.2).
Immunogen Sequence: AHTFS
Tissue Specificity Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T-cells from patients with atopic dermatitis.
Post Translational Modifications Phosphorylated by AKT1.insulin-induced. Also phosphorylated by AMPK in response to insulin. Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation. Has no effect on SLC2A4/GLUT4 internalization. Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients.
Function May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
Protein Name Tbc1 Domain Family Member 4
Akt Substrate Of 160 Kda
As160
Database Links Reactome: R-HSA-1445148
Cellular Localisation Cytoplasm
Isoform 2 Shows A Cytoplasmic Perinuclear Localization In A Myoblastic Cell Line In Resting And Insulin-Stimulated Cells
Alternative Antibody Names Anti-Tbc1 Domain Family Member 4 antibody
Anti-Akt Substrate Of 160 Kda antibody
Anti-As160 antibody
Anti-TBC1D4 antibody
Anti-AS160 antibody
Anti-KIAA0603 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance