• Immunohistochemistry analysis of paraffin-embedded human brain, using STK39 (Phospho-Ser325) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-STK39-Ser323 antibody (291-340 aa) (STJ91219)

SKU:
STJ91219

Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-STE20/SPS1-related proline-alanine-rich protein kinase-Ser323 (291-340 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STK39
Gene ID: 27347
Uniprot ID: STK39_HUMAN
Immunogen Region: 291-340 aa
Specificity: Phospho-SPAK (S325) Polyclonal Antibody detects endogenous levels of SPAK protein only when phosphorylated at S325.
Immunogen: The antiserum was produced against synthesized peptide derived from the human STK39 around the phosphorylation site of Ser325 at the amino acid range 291-340
Post Translational Modifications Phosphorylation at Thr-231 by WNK kinases (WNK1, WNK2, WNK3 or WNK4) is required for activation. Autophosphorylation at Thr-231 positively regulates its activity. Phosphorylated at Ser-309 by PRKCQ.
Function Effector serine/threonine-protein kinase component of the WNK-SPAK/OSR1 kinase cascade, which is involved in various processes, such as ion transport, response to hypertonic stress and blood pressure. Specifically recognizes and binds proteins with a RFXV motif. Acts downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4): following activation by WNK kinases, catalyzes phosphorylation of ion cotransporters, such as SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A5/KCC2 or SLC12A6/KCC3, regulating their activity. Mediates regulatory volume increase in response to hyperosmotic stress by catalyzing phosphorylation of ion cotransporters SLC12A1/NKCC2, SLC12A2/NKCC1 and SLC12A6/KCC3 downstream of WNK1 and WNK3 kinases. Phosphorylation of Na-K-Cl cotransporters SLC12A2/NKCC1 and SLC12A2/NKCC1 promote their activation and ion influx.simultaneously, phosphorylation of K-Cl cotransporters SLC12A5/KCC2 and SLC12A6/KCC3 inhibit their activity, blocking ion efflux. Acts as a regulator of NaCl reabsorption in the distal nephron by mediating phosphorylation and activation of the thiazide-sensitive Na-Cl cotransporter SLC12A3/NCC in distal convoluted tubule cells of kidney downstream of WNK4. Mediates the inhibition of SLC4A4, SLC26A6 as well as CFTR activities. Phosphorylates RELT.
Protein Name Ste20/Sps1-Related Proline-Alanine-Rich Protein Kinase
Ste-20-Related Kinase
Dcht
Serine/Threonine-Protein Kinase 39
Cellular Localisation Cytoplasm
Nucleus
Nucleus When Caspase-Cleaved
Alternative Antibody Names Anti-Ste20/Sps1-Related Proline-Alanine-Rich Protein Kinase antibody
Anti-Ste-20-Related Kinase antibody
Anti-Dcht antibody
Anti-Serine/Threonine-Protein Kinase 39 antibody
Anti-STK39 antibody
Anti-PASK antibody
Anti-SPAK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance