• Western blot analysis of extracts of various cell lines, using Phospho-STK3-T384 antibody (STJ11101069) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of various lysates using Phospho-STK3/MST2-T384 Rabbit polyclonal antibody (STJ11101069) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-STK3-T384 antibody (STJ11101069)

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STJ11101069

$174.83 - $863.33
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-STK3-T384 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STK3
Gene ID: 6788
Uniprot ID: STK3_HUMAN
Immunogen: A synthetic phosphorylated peptide around T384 of human STK3/MST2 (NP_006272.2).
Immunogen Sequence: NATSP
Tissue Specificity Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues.
Post Translational Modifications Phosphorylation at Thr-117 and Thr-384 by PKB/AKT1, leads to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation, and increase in its binding to RAF1. Proteolytically cleaved by caspase-3 during apoptosis. Proteolytic cleavage results in kinase activation and nuclear translocation of the truncated form (MST1/N).
Function Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1. Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38.
Protein Name Serine/Threonine-Protein Kinase 3
Mammalian Ste20-Like Protein Kinase 2
Mst-2
Ste20-Like Kinase Mst2
Serine/Threonine-Protein Kinase Krs-1 Cleaved Into - Serine/Threonine-Protein Kinase 3 36kda Subunit
Mst2/N - Serine/Threonine-Protein Kinase 3 20kda Subunit
Mst2/C
Database Links Reactome: R-HSA-2028269
Cellular Localisation Cytoplasm
Nucleus
The Caspase-Cleaved Form Cycles Between Nucleus And Cytoplasm
Phosphorylation At Thr-117 Leads To Inhibition Of Nuclear Translocation
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase 3 antibody
Anti-Mammalian Ste20-Like Protein Kinase 2 antibody
Anti-Mst-2 antibody
Anti-Ste20-Like Kinase Mst2 antibody
Anti-Serine/Threonine-Protein Kinase Krs-1 Cleaved Into - Serine/Threonine-Protein Kinase 3 36kda Subunit antibody
Anti-Mst2/N - Serine/Threonine-Protein Kinase 3 20kda Subunit antibody
Anti-Mst2/C antibody
Anti-STK3 antibody
Anti-KRS1 antibody
Anti-MST2 antibody

Information sourced from Uniprot.org

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