Anti-Phospho-SMC1A-Ser957 antibody (931-980 aa) (STJ90406)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human SMC1 around the phosphorylation site of Ser957 at the amino acid range 931-980
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Structural maintenance of chromosomes protein 1A-Ser957 (931-980 aa) for use in WB, IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SMC1A |
| Gene ID | 8243 |
| Uniprot ID | SMC1A_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human SMC1 around the phosphorylation site of Ser957 at the amino acid range 931-980 |
| Immunogen Region | 931-980 aa |
| Specificity | Phospho-SMC1 (S957) Polyclonal Antibody detects endogenous levels of SMC1 protein only when phosphorylated at S957. |
Additional Info
| Function | Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint. |
| Protein Name | Structural Maintenance Of Chromosomes Protein 1aSmc Protein 1aSmc-1-AlphaSmc-1aSb1.8 |
| Database Links | Reactome: R-HSA-1221632Reactome: R-HSA-2467813Reactome: R-HSA-2468052Reactome: R-HSA-2470946Reactome: R-HSA-2500257Reactome: R-HSA-3108214Reactome: R-HSA-9018519 |
| Cellular Localisation | NucleusChromosomeCentromereKinetochoreAssociates With ChromatinBefore Prophase It Is Scattered Along Chromosome ArmsDuring ProphaseMost Of Cohesin Complexes Dissociate From Chromatin Probably Because Of Phosphorylation By PlkExcept At CentromeresWhere Cohesin Complexes RemainAt AnaphaseThe Rad21 Subunit Of The Cohesin Complex Is CleavedLeading To The Dissociation Of The Complex From ChromosomesAllowing Chromosome SeparationIn Germ CellsCohesin Complex Dissociates From Chromatin At Prophase IAnd May Be Replaced By A Meiosis-Specific Cohesin ComplexThe Phosphorylated Form On Ser-957 And Ser-966 Associates With Chromatin During G1/S/G2 Phases But Not During M PhaseSuggesting That Phosphorylation Does Not Regulate Cohesin FunctionIntegral Component Of The Functional Centromere-Kinetochore Complex At The Kinetochore Region During Mitosis |
| Alternative Antibody Names | Anti-Structural Maintenance Of Chromosomes Protein 1a antibodyAnti-Smc Protein 1a antibodyAnti-Smc-1-Alpha antibodyAnti-Smc-1a antibodyAnti-Sb1.8 antibodyAnti-SMC1A antibodyAnti-DXS423E antibodyAnti-KIAA0178 antibodyAnti-SB1.8 antibodyAnti-SMC1 antibodyAnti-SMC1L1 antibody |
Information sourced from Uniprot.org