• Western blot analysis of lysates from HeLa cells treated with EGF, using Paxillin (Phospho-Tyr118) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-Paxillin (Y118) Polyclonal Antibody

Anti-Phospho-PXN-Tyr118 antibody (85-134 aa) (STJ90379)

SKU:
STJ90379

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Paxillin-Tyr118 (85-134 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PXN
Gene ID: 5829
Uniprot ID: PAXI_HUMAN
Immunogen Region: 85-134 aa
Specificity: Phospho-Paxillin (Y118) Polyclonal Antibody detects endogenous levels of Paxillin protein only when phosphorylated at Y118.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Paxillin around the phosphorylation site of Tyr118 at the amino acid range 85-134
Function Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Recruits other proteins such as TRIM15 to focal adhesion.
Protein Name Paxillin
Database Links Reactome: R-HSA-180292
Reactome: R-HSA-4420097
Reactome: R-HSA-445355
Reactome: R-HSA-446343
Reactome: R-HSA-446388
Reactome: R-HSA-8849471
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Junction
Focal Adhesion
Cell Cortex
Colocalizes With Integrins At The Cell Periphery
Colocalize With Pxn To Membrane Ruffles And The Leading Edge Of Migrating Cells
Alternative Antibody Names Anti-Paxillin antibody
Anti-PXN antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance