• Western blot analysis of extracts of HeLa and RAW 264. 7 cells, using Phospho-SHP1-Y564 antibody (STJ117885) at 1:2000 dilution. HeLa cell lysates were treated by CIP at 37°C for 1 hour. RAW 264. 7 cell lysates were treated by CIP at 37°C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Western blot analysis of lysates from HeLa and RAW 264. 7 cells, using Phospho-SHP1-Y564 Rabbit polyclonal antibody (STJ117885) at 1:2000 dilution. HeLa cell lysates were treated by CIP at 37°C for 1 hour. RAW 264. 7 cell lysates were treated by CIP at 37°C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-Phospho-PTPN6-Y564 antibody (STJ117885)

SKU:
STJ117885

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-PTPN6-Y564 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTPN6
Gene ID: 5777
Uniprot ID: PTN6_HUMAN
Immunogen: A synthetic phosphorylated peptide around Y564 of human PTPN6 (NP_002822.2).
Immunogen Sequence: DVYEN
Tissue Specificity Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
Post Translational Modifications Phosphorylated on tyrosine residues. Binding of KITLG/SCF to KIT increases tyrosine phosphorylation. Phosphorylation at Tyr-564 enhances phosphatase activity.
Function Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.
Protein Name Tyrosine-Protein Phosphatase Non-Receptor Type 6
Hematopoietic Cell Protein-Tyrosine Phosphatase
Protein-Tyrosine Phosphatase 1c
Ptp-1c
Protein-Tyrosine Phosphatase Shp-1
Sh-Ptp1
Database Links Reactome: R-HSA-114604
Reactome: R-HSA-1433559
Reactome: R-HSA-201556
Reactome: R-HSA-210990
Reactome: R-HSA-388841
Reactome: R-HSA-389948
Reactome: R-HSA-391160
Reactome: R-HSA-432142
Reactome: R-HSA-512988
Reactome: R-HSA-5690714
Reactome: R-HSA-6798695
Reactome: R-HSA-877300
Reactome: R-HSA-877312
Reactome: R-HSA-9008059
Reactome: R-HSA-909733
Reactome: R-HSA-912526
Reactome: R-HSA-912694
Reactome: R-HSA-9705671
Reactome: R-HSA-982772
Reactome: R-HSA-983695
Cellular Localisation Cytoplasm
Nucleus
In Neurons
Translocates Into The Nucleus After Treatment With Angiotensin Ii
Shuttles Between The Cytoplasm And Nucleus Via Its Association With Pdpk1
Alternative Antibody Names Anti-Tyrosine-Protein Phosphatase Non-Receptor Type 6 antibody
Anti-Hematopoietic Cell Protein-Tyrosine Phosphatase antibody
Anti-Protein-Tyrosine Phosphatase 1c antibody
Anti-Ptp-1c antibody
Anti-Protein-Tyrosine Phosphatase Shp-1 antibody
Anti-Sh-Ptp1 antibody
Anti-PTPN6 antibody
Anti-HCP antibody
Anti-PTP1C antibody

Information sourced from Uniprot.org

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