• Immunohistochemistry analysis of paraffin-embedded human brain, using SHP-2 (Phospho-Tyr580) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from A431 cells, using SHP-2 (Phospho-Tyr580) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-SH-PTP2 (Y580) Polyclonal Antibody

Anti-Phospho-PTPN11-Tyr580 antibody (546-595 aa) (STJ90742)

SKU:
STJ90742

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Tyrosine-protein phosphatase non-receptor type 11-Tyr580 (546-595 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTPN11
Gene ID: 5781
Uniprot ID: PTN11_HUMAN
Immunogen Region: 546-595 aa
Specificity: Phospho-SH-PTP2 (Y580) Polyclonal Antibody detects endogenous levels of SH-PTP2 protein only when phosphorylated at Y580.
Immunogen: The antiserum was produced against synthesized peptide derived from the human SHP-2 around the phosphorylation site of Tyr580 at the amino acid range 546-595
Post Translational Modifications Phosphorylated on Tyr-542 and Tyr-580 upon receptor protein tyrosine kinase activation.which creates a binding site for GRB2 and other SH2-containing proteins. Phosphorylated upon activation of the receptor-type kinase FLT3. Phosphorylated upon activation of the receptor-type kinase PDGFRA. Phosphorylated by activated PDGFRB.
Function Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway. Dephosphorylates GAB1, ARHGAP35 and EGFR. Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity. Dephosphorylates CDC73. Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification. Dephosphorylates tyrosine-phosphorylated NEDD9/CAS-L.
Protein Name Tyrosine-Protein Phosphatase Non-Receptor Type 11
Protein-Tyrosine Phosphatase 1d
Ptp-1d
Protein-Tyrosine Phosphatase 2c
Ptp-2c
Sh-Ptp2
Shp-2
Shp2
Sh-Ptp3
Database Links Reactome: R-HSA-1059683
Reactome: R-HSA-109704
Reactome: R-HSA-110056
Reactome: R-HSA-112411
Reactome: R-HSA-114604
Reactome: R-HSA-1170546
Reactome: R-HSA-1257604
Reactome: R-HSA-1295596
Reactome: R-HSA-1433557
Reactome: R-HSA-180292
Reactome: R-HSA-186763
Reactome: R-HSA-210990
Reactome: R-HSA-210993
Reactome: R-HSA-2219530
Reactome: R-HSA-2586552
Reactome: R-HSA-388841
Reactome: R-HSA-389513
Reactome: R-HSA-389948
Reactome: R-HSA-391160
Reactome: R-HSA-418886
Reactome: R-HSA-432142
Reactome: R-HSA-512988
Reactome: R-HSA-5654689
Reactome: R-HSA-5654693
Reactome: R-HSA-5654695
Reactome: R-HSA-5654700
Reactome: R-HSA-5654706
Reactome: R-HSA-5654710
Reactome: R-HSA-5654712
Reactome: R-HSA-5654720
Reactome: R-HSA-5654726
Reactome: R-HSA-5654727
Reactome: R-HSA-5654732
Reactome: R-HSA-5654733
Reactome: R-HSA-6811558
Reactome: R-HSA-877312
Reactome: R-HSA-8853659
Reactome: R-HSA-8854691
Reactome: R-HSA-8865999
Reactome: R-HSA-8934593
Reactome: R-HSA-9008059
Reactome: R-HSA-9028731
Reactome: R-HSA-909733
Reactome: R-HSA-912694
Reactome: R-HSA-936964
Reactome: R-HSA-9607240
Reactome: R-HSA-9645135
Reactome: R-HSA-9674555
Reactome: R-HSA-9680350
Reactome: R-HSA-9702518
Reactome: R-HSA-9703648
Reactome: R-HSA-9705671
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Tyrosine-Protein Phosphatase Non-Receptor Type 11 antibody
Anti-Protein-Tyrosine Phosphatase 1d antibody
Anti-Ptp-1d antibody
Anti-Protein-Tyrosine Phosphatase 2c antibody
Anti-Ptp-2c antibody
Anti-Sh-Ptp2 antibody
Anti-Shp-2 antibody
Anti-Shp2 antibody
Anti-Sh-Ptp3 antibody
Anti-PTPN11 antibody
Anti-PTP2C antibody
Anti-SHPTP2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance