Anti-Phospho-Profilin-Tyr-138 antibody (STJA0041357)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenPhospho-Profilin (Ser-138) synthetic peptide (coupled to carrier protein) that includes amino acids surrounding Ser-138 in human Profilin-1. This sequence is well conserved in rat and mouse Profilin-1. The serine site has some homology to the conserv
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Profilin-Tyr-138 for use in ELISA and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Antigen Affinity Purified |
| Dilution Range | WB 1:1000 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Storage at-20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20°C. |
Target Information
| Gene Symbol | PFN1 |
| Gene ID | 5216 |
| Uniprot ID | PROF1_HUMAN |
| Immunogen | Phospho-Profilin (Ser-138) synthetic peptide (coupled to carrier protein) that includes amino acids surrounding Ser-138 in human Profilin-1. This sequence is well conserved in rat and mouse Profilin-1. The serine site has some homology to the conserv |
| Immunogen Sequence | Human |
| Specificity | This antibody was cross-adsorbed to unphosphorylated Profilin (Ser-138) then affinity purified using Profilin (Ser-138) peptide (without carrier). The antibody detects a 15 kDa band corresponding to the apparent molecular mass of Profilin-1 in human |
Additional Info
| Tissue Specificity | Expressed in epididymis (at protein level). |
| Post Translational Modifications | Phosphorylation at Ser-138 reduces its affinity for G-actin and blocks its interaction with HTT, reducing its ability to inhibit androgen receptor (AR) and HTT aggregation. |
| Function | Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR. |
| Protein Name | Profilin-1Epididymis Tissue Protein Li 184aProfilin I |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-376176Reactome: R-HSA-4086400Reactome: R-HSA-5663220 |
| Cellular Localisation | CytoplasmCytoskeleton |
| Alternative Antibody Names | Anti-Profilin-1 antibodyAnti-Epididymis Tissue Protein Li 184a antibodyAnti-Profilin I antibodyAnti-PFN1 antibody |
Information sourced from Uniprot.org