• Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PKCalpha/beta II-T638/641 polyclonal antibody (STJ22341) at 1:1000 dilution or PRKCA antibody (A13342). NIH/3T3 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of methanol-fixed MCF-7 cells using Phospho-PKCalpha/beta II-T638/641 Rabbit polyclonal antibody (STJ22341).
  • Immunohistochemistry of paraffin-embedded human lung carcinoma tissue, using Phospho-PKCalpha/beta II-T638/641 antibody (STJ22341).
  • Immunohistochemistry analysis of Phospho-PKCalpha/beta II-T638/641 in paraffin-embedded human lung carcinoma tissue, using Phospho-PKCalpha/beta II-T638/641 Rabbit polyclonal antibody (STJ22341). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of methanol-fixed MCF-7 cells using Phospho-PRKCB-T641 antibody (STJ22341).
  • Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PKCalpha/beta II-T638/641 polyclonal antibody (STJ22341) at 1:1000 dilution or PRKCA antibody (A13342). NIH/3T3 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-PRKCA/PRKCB-T638/641 antibody (STJ22341)

SKU:
STJ22341

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-PKCalpha/beta II-T638/641 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic phosphorylated peptide around T638 of human PKCalpha/beta IIPRKCA (NP_002728.2).
Immunogen Sequence: VLTPP
Background Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in tandem in a gene cluster on chromosome 19. The encoded protein may be involved in proteolytic cascade in the skin and may serve as a biomarker for ovarian cancer. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.

Information sourced from Uniprot.org

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