• Western blot analysis of lysates from COS7 cells treated with PMA 125ng/ml 30', using PKA-R2 beta (Phospho-Ser113) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-PRKAR2B-Ser113 antibody (79-128 aa) (STJ90520)

SKU:
STJ90520

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-cAMP-dependent protein kinase type II-beta regulatory subunit-Ser113 (79-128 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRKAR2B
Gene ID: 5577
Uniprot ID: KAP3_HUMAN
Immunogen Region: 79-128 aa
Specificity: Phospho-PKA II Beta reg (S113) Polyclonal Antibody detects endogenous levels of PKA II Beta reg protein only when phosphorylated at S113.
Immunogen: The antiserum was produced against synthesized peptide derived from the human PKA-R2 beta around the phosphorylation site of Ser113 at the amino acid range 79-128
Post Translational Modifications Phosphorylated by the activated catalytic chain.
Function Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
Protein Name Camp-Dependent Protein Kinase Type Ii-Beta Regulatory Subunit
Database Links Reactome: R-HSA-163615
Reactome: R-HSA-164378
Reactome: R-HSA-180024
Reactome: R-HSA-2565942
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-381676
Reactome: R-HSA-432040
Reactome: R-HSA-442720
Reactome: R-HSA-5610787
Reactome: R-HSA-5620912
Reactome: R-HSA-8854518
Reactome: R-HSA-9634597
Reactome: R-HSA-9660821
Reactome: R-HSA-9664323
Reactome: R-HSA-983231
Cellular Localisation Cytoplasm
Cell Membrane
Colocalizes With Pja2 In The Cytoplasm And At The Cell Membrane
Alternative Antibody Names Anti-Camp-Dependent Protein Kinase Type Ii-Beta Regulatory Subunit antibody
Anti-PRKAR2B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance