• Western blot analysis of extracts of various cells, using Phospho-PPP2CA/PPP2CB-Y307 antibody (STJ11101061) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of various lysates using Phospho-PPP2CA/PPP2CB-Y307 Rabbit polyclonal antibody (STJ11101061) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-PPP2CA/PPP2CB-Y307 antibody (STJ11101061)

SKU:
STJ11101061

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-PPP2CA/PPP2CB-Y307 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic phosphorylated peptide around Y307 of human PPP2CA/PPP2CBPPP2CB (NP_001009552.1).
Immunogen Sequence: PDYFL
Background This gene is a member of the mitochondrial carrier subfamily of solute carrier protein genes. The product of this gene functions as a gated pore that translocates ADP from the cytoplasm into the mitochondrial matrix and ATP from the mitochondrial matrix into the cytoplasm. The protein is implicated in the function of the permability transition pore complex (PTPC) , which regulates the release of mitochondrial products that induce apoptosis. The human genome contains several non-transcribed pseudogenes of this gene.

Information sourced from Uniprot.org

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