• Western blot analysis of extracts of various cell lines, using Phospho-PDHA1-S293 rabbit monoclonal antibody (STJ11102604) at 1:1000 dilution. Hela cells and NIH/3T3 cells and C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3s.
  • Western blot analysis of extracts of HeLa cells, using Phospho-PDHA1-S293 antibody (STJ11102604) at 1:115000 dilution. 293T cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of HeLa cells, using Phospho-PDHA1-S293 antibody (STJ11102604) at1:20000 dilution. NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of NIH/3T3 using Phospho-PDHA1-S293 rabbit monoclonal antibody (STJ11102604) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300 Mu g extracts of NIH/3T3 cells using 3 Mu g Phospho-PDHA1-S293 antibody (STJ11102604). Western blot was performed from the immunoprecipitate using Phospho-PDHA1-S293 antibody (STJ11102604) at a dilution of 1:1000.

Anti-Phospho-PDHA1-S293 antibody [S4MR] (STJ11102604)

SKU:
STJ11102604

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Phospho-PDHA1-S293 is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDHA1
Gene ID: 5160
Uniprot ID: ODPA_HUMAN
Immunogen: A synthetic phosphorylated peptide around S293 of human PDHA1 (P08559).
Immunogen Sequence: LMELQTYRYHGHSMSDPGVS YRTREEIQE
Tissue Specificity Ubiquitous.
Post Translational Modifications Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme.for this phosphorylation at a single site is sufficient. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation. Acetylation alters the phosphorylation pattern. Deacetylated by SIRT3.
Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Protein Name Pyruvate Dehydrogenase E1 Component Subunit Alpha - Somatic Form - Mitochondrial
Pdhe1-A Type I
Database Links Reactome: R-HSA-204174
Reactome: R-HSA-389661
Reactome: R-HSA-5362517
Reactome: R-HSA-70268
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Pyruvate Dehydrogenase E1 Component Subunit Alpha - Somatic Form - Mitochondrial antibody
Anti-Pdhe1-A Type I antibody
Anti-PDHA1 antibody
Anti-PHE1A antibody

Information sourced from Uniprot.org

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