Western blot analysis of lysates from HeLa cells, using Phospho-PDHA1-S293 Rabbit mAb (STJ11102604) at 1:23000 dilution. 293T cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-PDHA1-S293 antibody [S4MR] (STJ11102604)

SKU:
STJ11102604
€183.13 - €803.13
Free Shipping
Processing The item has been added
Enquire For Bulk Order

Covered by St John's Laboratory Guarantee

Host: Rabbit
Applications: WB/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:2000-1:20000
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDHA1
Gene ID: 5160
Uniprot ID: ODPA_HUMAN
Specificity: A synthetic phosphorylated peptide around S293 of human PDHA1 (P08559).
Immunogen Sequence: GHSMSD
Tissue Specificity Ubiquitous.
Post Translational Modifications Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme.for this phosphorylation at a single site is sufficient. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation. Acetylation alters the phosphorylation pattern. Deacetylated by SIRT3.
Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Protein Name Pyruvate Dehydrogenase E1 Component Subunit Alpha - Somatic Form - Mitochondrial
Pdhe1-A Type I
Database Links Reactome: R-HSA-204174
Reactome: R-HSA-5362517
Reactome: R-HSA-9837999
Reactome: R-HSA-9861559
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Pyruvate Dehydrogenase E1 Component Subunit Alpha - Somatic Form - Mitochondrial antibody
Anti-Pdhe1-A Type I antibody
Anti-PDHA1 antibody
Anti-PHE1A antibody

Information sourced from Uniprot.org

Related Products

Model Name Host Clonality Reactivity Application
STJ11103767 Anti-Phospho-PDHA1-S293 antibody (STJ11103767) Rabbit Polyclonal Human/Mouse/Rat WB/ELISA
STJ11105820 Anti-Phospho-PDHA1-S293 antibody [S5820RM] (STJ11105820) Rabbit Monoclonal Human/Mouse WB/ELISA
STJ13101309 Anti-Phospho-LIMK2-S291/S293 antibody (STJ13101309) Rabbit Polyclonal Mouse/Rat/Human IHC/WB
STJ13101312 Anti-Phospho-LIMK2-S291/S293 antibody (STJ13101312) Sheep Polyclonal Mouse/Rat/Human IHC/WB
STJ13101311 Anti-Phospho-LIMK2-S291/S293 antibody (STJ13101311) Sheep Polyclonal Mouse/Rat/Human IHC/WB
!