• Western blot of rat hippocampal lysate showing specific immunolabeling of the ~68 kDa to ~70 kDa PAK protein phosphorylated at Ser402 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 minutes).

Anti-Phospho-PAK1-3-Thr402 antibody (STJA0003739)

SKU:
STJA0003739-100

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat/Bovine/Canine/Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-PAK-1, 2, 3-Thr402 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:1000
IHC
ICC
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Pak1
Gene ID: 29431
Uniprot ID: PAK1_RAT
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr402 conjugated to KLH.
Tissue Specificity Expressed predominantly in the brain, with higher expression in neuronal groups associated with motor function, and at lower levels in the spleen.
Post Translational Modifications Autophosphorylated in trans, meaning that in a dimer, one kinase molecule phosphorylates the other one. Activated by autophosphorylation at Thr-422 in response to a conformation change, triggered by interaction with GTP-bound CDC42 or RAC1. Activated by phosphorylation at Thr-422 by PDPK1. Phosphorylated by JAK2 in response to PRL.this increases PAK1 kinase activity. Phosphorylated at Ser-21 by PKB/AKT.this reduces interaction with NCK1 and association with focal adhesion sites. Activated by phosphorylation at Thr-422 by BRSK2. Upon DNA damage, phosphorylated at Thr-211 and translocates to the nucleoplasm. Phosphorylated at tyrosine residues, which can be enhanced by NTN1.
Function Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. Can directly phosphorylate BAD and protects cells against apoptosis. Activated by interaction with CDC42 and RAC1. Functions as GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway. Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases. Involved in the reorganization of the actin cytoskeleton, actin stress fibers and of focal adhesion complexes. Phosphorylates the tubulin chaperone TBCB and thereby plays a role in the regulation of microtubule biogenesis and organization of the tubulin cytoskeleton. Plays a role in the regulation of insulin secretion in response to elevated glucose levels. Part of a ternary complex that contains PAK1, DVL1 and MUSK that is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. Phosphorylates MYL9/MLC2. Phosphorylates RAF1 at 'Ser-338' and 'Ser-339' resulting in: activation of RAF1, stimulation of RAF1 translocation to mitochondria, phosphorylation of BAD by RAF1, and RAF1 binding to BCL2. Phosphorylates SNAI1 at 'Ser-246' promoting its transcriptional repressor activity by increasing its accumulation in the nucleus. In podocytes, promotes NR3C2 nuclear localization. Required for atypical chemokine receptor ACKR2-induced phosphorylation of LIMK1 and cofilin (CFL1) and for the up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3, maybe through CFL1 phosphorylation and inactivation. Plays a role in RUFY3-mediated facilitating gastric cancer cells migration and invasion. In response to DNA damage, phosphorylates MORC2 which activates its ATPase activity and facilitates chromatin remodeling. In neurons, plays a crucial role in regulating GABA(A) receptor synaptic stability and hence GABAergic inhibitory synaptic transmission through its role in F-actin stabilization. In hippocampal neurons, necessary for the formation of dendritic spines and excitatory synapses.this function is dependent on kinase activity and may be exerted by the regulation of actomyosin contractility through the phosphorylation of myosin II regulatory light chain (MLC). Along with GIT1, positively regulates microtubule nucleation during interphase. Phosphorylates FXR1, promoting its localization to stress granules and activity.
Protein Name Serine/Threonine-Protein Kinase Pak 1
Alpha-Pak
Protein Kinase Muk2
P21-Activated Kinase 1
Pak-1
P68-Pak
Database Links Reactome: R-RNO-202433
Reactome: -RNO-2029482
Reactome: -RNO-2871796
Reactome: -RNO-376172
Reactome: -RNO-389359
Reactome: -RNO-3928662
Reactome: -RNO-3928664
Reactome: -RNO-399954
Reactome: -RNO-445144
Reactome: -RNO-445355
Reactome: -RNO-5218920
Reactome: -RNO-5621575
Reactome: -RNO-5627123
Reactome: -RNO-5687128
Reactome: -RNO-8964616
Reactome: -RNO-9013149
Reactome: -RNO-9013404
Reactome: -RNO-9013406
Reactome: -RNO-9013407
Reactome: -RNO-9013420
Reactome: -RNO-9013423
Reactome: -RNO-9013424
Cellular Localisation Cytoplasm
Cell Junction
Focal Adhesion
Cell Projection
Lamellipodium
Cell Membrane
Ruffle Membrane
Invadopodium
Nucleus
Nucleoplasm
Chromosome
Cytoskeleton
Microtubule Organizing Center
Centrosome
Recruited To The Cell Membrane By Interaction With Cdc42 And Rac1
Recruited To Focal Adhesions Upon Activation
Colocalized With Cib1 Within Membrane Ruffles During Cell Spreading Upon Readhesion To Fibronectin
Colocalizes With Rufy3
F-Actin And Other Core Migration Components In Invadopodia At The Cell Periphery
Upon Dna Damage
Translocates To The Nucleoplasm When Phosphorylated At Thr-212 Where Is Co-Recruited With Morc2 On Damaged Chromatin
Localization To The Centrosome Does Not Depend Upon The Presence Of Gamma-Tubulin
Localization Of The Active
But Not Inactive
Protein To The Adhesions And Edge Of Lamellipodia Is Mediated By Interaction With Git1
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Pak 1 antibody
Anti-Alpha-Pak antibody
Anti-Protein Kinase Muk2 antibody
Anti-P21-Activated Kinase 1 antibody
Anti-Pak-1 antibody
Anti-P68-Pak antibody
Anti-Pak1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance