• Western blot analysis of extracts of various cell lines, using Phospho-NEDD4L-S448 antibody (STJ11100984) at 1:1000 dilution. NIH 3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. C6 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of lysates from 293T cells, using Phospho-NEDD4L-S448 Rabbit polyclonal antibody (A8085). 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of extracts of 293T cells, using Phospho-NEDD4L-S448 polyclonal antibody (STJ11100984) at 1:1000 dilution or NEDD4L antibody (A8085). 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of various lysates using Phospho-NEDD4L-S448 Rabbit polyclonal antibody (STJ11100984) at 1:1000 dilution. NIH 3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. C6 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-Phospho-NEDD4L-S448 antibody (STJ11100984)

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STJ11100984

£129.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-NEDD4L-S448 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NEDD4L
Gene ID: 23327
Uniprot ID: NED4L_HUMAN
Immunogen: A synthetic phosphorylated peptide around S448 of human NEDD4L (NP_001138439.1).
Immunogen Sequence: SLSSP
Tissue Specificity Ubiquitously expressed, with highest levels in prostate, pancreas, and kidney. Expressed in melanocytes.
Post Translational Modifications Phosphorylated by SGK1 or PKA.which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation. Auto-ubiquitinated. Deubiquitinated by USP36, no effect on NEDD4L protein levels. Both proteins interact and regulate each other's ubiquitination levels.
Function E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins and is thereby implicated in the regulation of various signaling pathways including autophagy, innate immunity or DNA repair. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Downregulates autophagy and cell growth by ubiquitinating and reducing cellular ULK1 or ASCT2 levels. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, SCN2A/Nav1.2, SCN3A/Nav1.3, SCN5A/Nav1.5, SCN9A/Nav1.7, SCN10A/Nav1.8, KCNA3/Kv1.3, KCNH2, EAAT1, KCNQ2/Kv7.2, KCNQ3/Kv7.3 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1. Plays a role in dendrite formation by melanocytes. Involved in the regulation of TOR signaling. Ubiquitinates and regulates protein levels of NTRK1 once this one is activated by NGF. Plays a role in antiviral innate immunity by catalyzing 'Lys-29'-linked cysteine ubiquitination of TRAF3, resulting in enhanced 'Lys-48' and 'Lys-63'-linked ubiquitination of TRAF3.
Protein Name E3 Ubiquitin-Protein Ligase Nedd4-Like
Hect-Type E3 Ubiquitin Transferase Ned4l
Nedd4.2
Nedd4-2
Database Links Reactome: R-HSA-162588
Reactome: R-HSA-2173788
Reactome: R-HSA-2173795
Reactome: R-HSA-2672351
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Golgi Apparatus
Endosome
Multivesicular Body
May Be Recruited To Exosomes By Ndfip1
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Nedd4-Like antibody
Anti-Hect-Type E3 Ubiquitin Transferase Ned4l antibody
Anti-Nedd4.2 antibody
Anti-Nedd4-2 antibody
Anti-NEDD4L antibody
Anti-KIAA0439 antibody
Anti-NEDL3 antibody

Information sourced from Uniprot.org

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