• Western blot analysis of lysates from PC12 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-NDRG1-Thr346 antibody (STJ194731)

SKU:
STJ194731

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Protein NDRG1-Thr346 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000-2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDRG1
Gene ID: 10397
Uniprot ID: NDRG1_HUMAN
Specificity: This antibody detects endogenous levels of Human Mouse Rat NDRG1 (phospho-Thr346)
Immunogen: Synthesized phosho peptide around human NDRG1 (Thr346)
Post Translational Modifications Under stress conditions, phosphorylated in the C-terminal on many serine and threonine residues. Phosphorylated in vitro by PKA. Phosphorylation enhanced by increased intracellular cAMP levels. Homocysteine induces dephosphorylation. Phosphorylation by SGK1 is cell cycle dependent.
Function Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Has a role in cell trafficking, notably of the Schwann cell, and is necessary for the maintenance and development of the peripheral nerve myelin sheath. Required for vesicular recycling of CDH1 and TF. May also function in lipid trafficking. Protects cells from spindle disruption damage. Functions in p53/TP53-dependent mitotic spindle checkpoint. Regulates microtubule dynamics and maintains euploidy.
Protein Name Protein Ndrg1
Differentiation-Related Gene 1 Protein
Drg-1
N-Myc Downstream-Regulated Gene 1 Protein
Nickel-Specific Induction Protein Cap43
Reducing Agents And Tunicamycin-Responsive Protein
Rtp
Rit42
Database Links Reactome: R-HSA-6803205
Cellular Localisation Cytoplasm
Cytosol
Cytoskeleton
Microtubule Organizing Center
Centrosome
Nucleus
Cell Membrane
Mainly Cytoplasmic But Differentially Localized To Other Regions
Associates With The Plasma Membrane In Intestinal Epithelia And Lactating Mammary Gland
Translocated To The Nucleus In A P53/Tp53-Dependent Manner
In Prostate Epithelium And Placental Chorion
Located In Both The Cytoplasm And In The Nucleus
No Nuclear Localization In Colon Epithelium Cells
In Intestinal Mucosa
Prostate And Renal Cortex
Located Predominantly Adjacent To Adherens Junctions
Cytoplasmic With Granular Staining In Proximal Tubular Cells Of The Kidney And Salivary Gland Ducts
Recruits To The Membrane Of Recycling/Sorting And Late Endosomes Via Binding To Phosphatidylinositol 4-Phosphate
Associates With Microtubules
Colocalizes With Tubg1 In The Centrosome
Cytoplasmic Location Increased With Hypoxia
Phosphorylated Form Found Associated With Centromeres During S-Phase Of Mitosis And With The Plasma Membrane
Alternative Antibody Names Anti-Protein Ndrg1 antibody
Anti-Differentiation-Related Gene 1 Protein antibody
Anti-Drg-1 antibody
Anti-N-Myc Downstream-Regulated Gene 1 Protein antibody
Anti-Nickel-Specific Induction Protein Cap43 antibody
Anti-Reducing Agents And Tunicamycin-Responsive Protein antibody
Anti-Rtp antibody
Anti-Rit42 antibody
Anti-NDRG1 antibody
Anti-CAP43 antibody
Anti-DRG1 antibody
Anti-RTP antibody

Information sourced from Uniprot.org

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