• Western blot analysis of lysates from HeLa cells using Phospho-MRE11 (Ser676) Rabbit polyclonal antibody (STJ11105909) at 1:800 dilution. HeLa cells were treated by Calyculin A (200 nM) at 37 °C for 18 hours after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-MRE11-Ser676 antibody (STJ11105909)

SKU:
STJ11105909

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Double-Strand Break Repair Protein Mre11-Ser676 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MRE11
Gene ID: 4361
Uniprot ID: MRE11_HUMAN
Immunogen: A synthetic phosphorylated peptide around S676 of human MRE11 (NP_005582.1).
Immunogen Sequence: IMSQS
Post Translational Modifications Ubiquitinated following DNA damage. Ubiquitination triggers interaction with UBQLN4, leading to MRE11 removal from chromatin and degradation by the proteasome.
Function Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
Protein Name Double-Strand Break Repair Protein Mre11
Double-Strand Break Repair Protein Mre11a
Meiotic Recombination 11 Homolog 1
Mre11 Homolog 1
Meiotic Recombination 11 Homolog A
Mre11 Homolog A
Database Links Reactome: R-HSA-1834949
Reactome: R-HSA-2559586
Reactome: R-HSA-3270619
Reactome: R-HSA-5685938
Reactome: R-HSA-5685939
Reactome: R-HSA-5685942
Reactome: R-HSA-5693548
Reactome: R-HSA-5693554
Reactome: R-HSA-5693565
Reactome: R-HSA-5693568
Reactome: R-HSA-5693571
Reactome: R-HSA-5693579
Reactome: R-HSA-5693607
Reactome: R-HSA-5693616
Reactome: R-HSA-6804756
Reactome: R-HSA-69473
Reactome: R-HSA-912446
Reactome: R-HSA-9701192
Reactome: R-HSA-9704331
Reactome: R-HSA-9704646
Reactome: R-HSA-9709570
Reactome: R-HSA-9709603
Cellular Localisation Nucleus
Chromosome
Telomere
Localizes To Discrete Nuclear Foci After Treatment With Genotoxic Agents
Alternative Antibody Names Anti-Double-Strand Break Repair Protein Mre11 antibody
Anti-Double-Strand Break Repair Protein Mre11a antibody
Anti-Meiotic Recombination 11 Homolog 1 antibody
Anti-Mre11 Homolog 1 antibody
Anti-Meiotic Recombination 11 Homolog A antibody
Anti-Mre11 Homolog A antibody
Anti-MRE11 antibody
Anti-HNGS1 antibody
Anti-MRE11A antibody

Information sourced from Uniprot.org

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