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Anti-Phospho-MLC-2-Thr17/S18 antibody (5-54 aa) (STJ91117)

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MLC-2 (Phospho-Thr17+Ser18) Antibody
Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-MLC-2-Thr17/S18 antibody (5-54 aa) (STJ91117)

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STJ91117

$62.10 - $305.10

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General Information
Product Properties
Target Information
Additional Info
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Host:	Rabbit
Applications:	IHC/IF/ELISA
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Phospho-Myosin regulatory light chain 12A and Myosin regulatory light chain 12B and Myosin regulatory light polypeptide 9-Thr17/S18 (5-54 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA re

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-2000 IHC 1:100-1:300 ELISA 1:10000 IF 1:50-200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	MYL12B MYL9 103910/10627 10398 10627<
Uniprot ID:	ML12B_HUMAN MYL9_HUMAN ML12A_HUMAN<
Immunogen Region:	5-54 aa
Specificity:	Phospho-MLC-2 (T17/S18) Polyclonal Antibody detects endogenous levels of MLC-2 protein only when phosphorylated at T17/S18.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human MLC-2 around the phosphorylation site of Thr17 and Ser18 at the amino acid range 5-54

Function	Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
Protein Name	Myosin Regulatory Light Chain 12a Epididymis Secretory Protein Li 24 Hel-S-24 Mlc-2b Myosin Rlc Myosin Regulatory Light Chain 2 - Nonsarcomeric Myosin Regulatory Light Chain Mrlc3
Database Links	Reactome: R-HSA-3928664 Reactome: R-HSA-445355
Alternative Antibody Names	Anti-Myosin Regulatory Light Chain 12a antibody Anti-Epididymis Secretory Protein Li 24 antibody Anti-Hel-S-24 antibody Anti-Mlc-2b antibody Anti-Myosin Rlc antibody Anti-Myosin Regulatory Light Chain 2 - Nonsarcomeric antibody Anti-Myosin Regulatory Light Chain Mrlc3 antibody Anti-MYL12A antibody Anti-MLCB antibody Anti-MRLC3 antibody Anti-RLC antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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