| Host: |
Rabbit |
| Applications: |
WB/ICC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-MeCP2-Ser-421 is suitable for use in Western Blot and Immunocytochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Antigen Affinity Purified |
| Dilution Range: |
WB 1:500IHCICC 1:250IP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Mecp2 |
| Gene ID: |
17257 |
| Uniprot ID: |
MECP2_MOUSE |
| Specificity: |
The antibody detects a 75 kDa* protein corresponding to the molecular mass of MeCP2 on SDS-PAGE immunoblots of human PC3 cells treated with calyculin A and in mouse brain tissue. These reactivities are not observed after lambda phosphatase treatment. |
| Immunogen: |
phospho-MeCP2 (Ser-421) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 421 in mouse MeCP2. This peptide sequence is highly conserved in rat and human MeCP2, and has low homology to other nuclear proteins. |
| Post Translational Modifications | Phosphorylated on Ser-421 by CaMK2 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation. Does not seem to be phosphorylated on Ser-421 in other tissues. |
| Function | Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC). |
| Protein Name | Methyl-Cpg-Binding Protein 2Mecp-2 ProteinMecp2 |
| Database Links | Reactome: R-MMU-9022692 Q9Z2D6-1 |
| Cellular Localisation | NucleusColocalized With Methyl-Cpg In The GenomeColocalized With Tbl1x To The Heterochromatin Foci |
| Alternative Antibody Names | Anti-Methyl-Cpg-Binding Protein 2 antibodyAnti-Mecp-2 Protein antibodyAnti-Mecp2 antibodyAnti-Mecp2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
