| Short Description : | Rabbit polyclonal anti-Phospho-mDia3-Ser-196 for use in ELISA and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | ELISA/WB |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Purification: | Antigen Affinity Purified |
| Dilution Range: | WB 1:1000 |
| Formulation: | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction: | Storage at-20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20°C. |
| Gene Symbol: | Diaph3 |
| Gene ID: | 56419 |
| Uniprot ID: | DIAP3_MOUSE |
| Immunogen: | Phospho-mDia3 (Ser-196) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-196 in human mDia3 (Diap2). This peptide sequence is identical in rat and mouse mDia3, and the site is well conserved in human mDia1/Diap1 |
| Immunogen Sequence: | Human |
| Specificity: | The antibody detects a 130 kDa* protein corresponding to the apparent molecular mass of mDia3 on SDS-PAGE immunoblots of human Jurkat treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment. The antibody may also |
| Post Translational Modifications | Ubiquitinated. |
| Function | Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers. Required for cytokinesis, stress fiber formation and transcriptional activation of the serum response factor. Binds to GTP-bound form of Rho and to profilin: acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity. |
| Protein Name | Protein Diaphanous Homolog 3Diaphanous-Related Formin-3Drf3P134mdia2Mdia2 |
| Database Links | Reactome: R-MMU-5663220Reactome: -MMU-8980692Reactome: -MMU-9013026Reactome: -MMU-9013106Reactome: -MMU-9013149Reactome: -MMU-9013404Reactome: -MMU-9013405Reactome: -MMU-9013406Reactome: -MMU-9013408Reactome: -MMU-9013423Reactome: -MMU-9035034 |
| Cellular Localisation | CytoplasmNucleusDuring MitosisCo-Localizes With The Actin-Rich Cleavage Furrow And With The Microtubule-Rich Central Spindle During CytokinesisShuttles Between The Cytoplasm And The Nucleus |
| Alternative Antibody Names | Anti-Protein Diaphanous Homolog 3 antibodyAnti-Diaphanous-Related Formin-3 antibodyAnti-Drf3 antibodyAnti-P134mdia2 antibodyAnti-Mdia2 antibodyAnti-Diaph3 antibodyAnti-Diap3 antibody |
Information sourced from Uniprot.org
