• Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
  • Western Blot analysis of 1 K562 treated with LPS, 2 K562, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-MCM2-Ser139 antibody (STJ196353)

SKU:
STJ196353

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-DNA replication licensing factor MCM2-Ser139 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MCM2
Gene ID: 4171
Uniprot ID: MCM2_HUMAN
Specificity: This antibody detects endogenous levels of Human Mouse Rat MCM2 (phospho-Ser139)
Immunogen: Synthesized phosho peptide around human MCM2 (Ser139)
Function Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis.
Protein Name Dna Replication Licensing Factor Mcm2
Minichromosome Maintenance Protein 2 Homolog
Nuclear Protein Bm28
Database Links Reactome: R-HSA-176187
Reactome: R-HSA-176974
Reactome: R-HSA-68867
Reactome: R-HSA-68949
Reactome: R-HSA-68962
Reactome: R-HSA-69052
Cellular Localisation Nucleus
Chromosome
Associated With Chromatin Before The Formation Of Nuclei And Detaches From It As Dna Replication Progresses
Alternative Antibody Names Anti-Dna Replication Licensing Factor Mcm2 antibody
Anti-Minichromosome Maintenance Protein 2 Homolog antibody
Anti-Nuclear Protein Bm28 antibody
Anti-MCM2 antibody
Anti-BM28 antibody
Anti-CCNL1 antibody
Anti-CDCL1 antibody
Anti-KIAA0030 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance