| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-EB3-Ser-162 is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Antigen Affinity Purified |
| Dilution Range: |
WB 1:1000IHCICCIP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MAPRE3 |
| Gene ID: |
22924 |
| Uniprot ID: |
MARE3_HUMAN |
| Specificity: |
The antibody detects a 32 kDa* protein corresponding to the apparent molecular mass of phosphorylated EB3 on SDS-PAGE immunoblots of mouse brain. This reactivity is significantly reduced after lambda phosphatase treatment. |
| Immunogen: |
Phospho-EB3 (Ser-162) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 162 in human EB3. This peptide sequence is highly conserved in mouse and rat EB3, and is not conserved in EB1 and EB2. |
| Tissue Specificity | Predominantly expressed in brain and muscle. |
| Function | Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules. |
| Protein Name | Microtubule-Associated Protein Rp/Eb Family Member 3Eb1 Protein Family Member 3Ebf3End-Binding Protein 3Eb3Rp3 |
| Cellular Localisation | CytoplasmCytoskeletonAssociated With The Microtubule NetworkDetected At The Plus End Of Microtubules |
| Alternative Antibody Names | Anti-Microtubule-Associated Protein Rp/Eb Family Member 3 antibodyAnti-Eb1 Protein Family Member 3 antibodyAnti-Ebf3 antibodyAnti-End-Binding Protein 3 antibodyAnti-Eb3 antibodyAnti-Rp3 antibodyAnti-MAPRE3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
