| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Dual specificity mitogen-activated protein kinase kinase 3-Ser218 (173-222 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:5000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MAP2K3 |
| Gene ID: | 5606 |
| Uniprot ID: | MP2K3_HUMAN |
| Immunogen Region: | 173-222 aa |
| Specificity: | Phospho-MEK-3 (S189) Polyclonal Antibody detects endogenous levels of MEK-3 protein only when phosphorylated at S189. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human MKK3 around the phosphorylation site of Ser189 at the amino acid range 173-222 |
| Post Translational Modifications | Autophosphorylated. Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases positively regulates the kinase activity. Phosphorylated by TAOK2. (Microbial infection) Yersinia YopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway. |
| Function | Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. |
| Protein Name | Dual Specificity Mitogen-Activated Protein Kinase Kinase 3 Map Kinase Kinase 3 Mapkk 3 Mapk/Erk Kinase 3 Mek 3 Stress-Activated Protein Kinase Kinase 2 Sapk Kinase 2 Sapkk-2 Sapkk2 |
| Database Links | Reactome: R-HSA-2559580 Reactome: R-HSA-450302 Reactome: R-HSA-5210891 P46734-3 |
| Alternative Antibody Names | Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 3 antibody Anti-Map Kinase Kinase 3 antibody Anti-Mapkk 3 antibody Anti-Mapk/Erk Kinase 3 antibody Anti-Mek 3 antibody Anti-Stress-Activated Protein Kinase Kinase 2 antibody Anti-Sapk Kinase 2 antibody Anti-Sapkk-2 antibody Anti-Sapkk2 antibody Anti-MAP2K3 antibody Anti-MEK3 antibody Anti-MKK3 antibody Anti-PRKMK3 antibody Anti-SKK2 antibody |
Information sourced from Uniprot.org