• Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-LIN28A-Ser200 antibody (STJ194717)

SKU:
STJ194717

Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Protein lin-28 homolog A-Ser200 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LIN28A
Gene ID: 79727
Uniprot ID: LN28A_HUMAN
Specificity: This antibody detects endogenous levels of Human LIN28A (phospho-Ser200)
Immunogen: Synthesized phosho peptide around human LIN28A (Ser200)
Function RNA-binding protein that inhibits processing of pre-let-7 miRNAs and regulates translation of mRNAs that control developmental timing, pluripotency and metabolism. Seems to recognize a common structural G-quartet (G4) feature in its miRNA and mRNA targets (Probable). 'Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in mRNA stabilization. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Suppressor of microRNA (miRNA) biogenesis, including that of let-7, miR107, miR-143 and miR-200c. Specifically binds the miRNA precursors (pre-miRNAs), recognizing an 5'-GGAG-3' motif found in pre-miRNA terminal loop, and recruits TUT4 and TUT7 uridylyltransferases. This results in the terminal uridylation of target pre-miRNAs. Uridylated pre-miRNAs fail to be processed by Dicer and undergo degradation. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state by preventing let-7-mediated differentiation of embryonic stem cells. Localized to the periendoplasmic reticulum area, binds to a large number of spliced mRNAs and inhibits the translation of mRNAs destined for the ER, reducing the synthesis of transmembrane proteins, ER or Golgi lumen proteins, and secretory proteins. Binds to and enhances the translation of mRNAs for several metabolic enzymes, such as PFKP, PDHA1 or SDHA, increasing glycolysis and oxidative phosphorylation. Which, with the let-7 repression may enhance tissue repair in adult tissue.
Protein Name Protein Lin-28 Homolog A
Lin-28a
Zinc Finger Cchc Domain-Containing Protein 1
Database Links Reactome: R-HSA-452723
Cellular Localisation Cytoplasm
Rough Endoplasmic Reticulum
P-Body
Stress Granule
Nucleus
Nucleolus
Predominantly Cytoplasmic
In The Cytoplasm
Localizes To Peri-Endoplasmic Reticulum Regions And Detected In The Microsomal Fraction Derived From Rough Endoplasmic Reticulum (Rer) Following Subcellular Fractionation
May Be Bound To The Cytosolic Surface Of Rer On Which Er-Associated Mrnas Are Translated
Shuttle From The Nucleus To The Cytoplasm Requires Rna-Binding
Nucleolar Localization Is Observed In 10-15% Of The Nuclei In Differentiated Myotubes
Alternative Antibody Names Anti-Protein Lin-28 Homolog A antibody
Anti-Lin-28a antibody
Anti-Zinc Finger Cchc Domain-Containing Protein 1 antibody
Anti-LIN28A antibody
Anti-CSDD1 antibody
Anti-LIN28 antibody
Anti-ZCCHC1 antibody

Information sourced from Uniprot.org

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