Anti-Phospho-LIMK2-S291/S293 antibody (STJ13101309)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from the phospho S291 & S293 region of human LIMK2 (LIM domain kinase 2, LIMK-2) conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in rat, mouse.
General Information
| Short Description | Rabbit polyclonal anti-Phospho-LIMK2-S291/S293 for use in IHC and WB in Mouse, Rat and Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Mouse/Rat/Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. This antibody may also recognise the unphosphorylated form. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20ยฐC for long term storage and refrigerated at 2-8ยฐC for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | Limk2 |
| Gene ID | 16886 |
| Uniprot ID | LIMK2_MOUSE |
| Immunogen | A synthetic peptide from the phospho S291 & S293 region of human LIMK2 (LIM domain kinase 2, LIMK-2) conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in rat, mouse. |
| Specificity | Specific for LIMK2. |
Additional Info
| Post Translational Modifications | Phosphorylated on serine and/or threonine residues by ROCK1. |
| Function | Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Involved in astral microtubule organization and mitotic spindle orientation during early stages of mitosis by mediating phosphorylation of TPPP. Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro. Suppresses ciliogenesis via multiple pathways.phosphorylation of CFL1, suppression of directional trafficking of ciliary vesicles to the ciliary base, and by facilitating YAP1 nuclear localization where it acts as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1. |
| Protein Name | Lim Domain Kinase 2Limk-2 |
| Database Links | |
| Cellular Localisation | CytoplasmCytoskeletonSpindleMicrotubule Organizing CenterCentrosomeIsoform Limk2a: CytoplasmNucleusIsoform Limk2b: CytoplasmPerinuclear Region |
| Alternative Antibody Names | Anti-Lim Domain Kinase 2 antibodyAnti-Limk-2 antibodyAnti-Limk2 antibody |
Information sourced from Uniprot.org