• Western blot analysis of extracts from K562 cells, using Phospho-Ob-R (Y1141) Polyclonal Antibody.

Anti-Phospho-LEPR-Tyr1141 antibody (STJ91360)

SKU:
STJ91360

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Leptin receptor-Tyr1141 is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LEPR
Gene ID: 3953
Uniprot ID: LEPR_HUMAN
Specificity: Phospho-Ob-R (Y1141) Polyclonal Antibody detects endogenous levels of Ob-R protein only when phosphorylated at Y1141.
Immunogen: Synthesized phospho-peptide around the phosphorylation site of human Ob-R (phospho Tyr1141)
Post Translational Modifications On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation,for interaction with SOCS3 and SOCS3 mediated inhibition of leptin signaling. Phosphorylation on Tyr-1141 is required for STAT3 binding/activation. Phosphorylation of Tyr-1079 has a more accessory role.
Function Receptor for hormone LEP/leptin (Probable). On ligand binding, mediates LEP central and peripheral effects through the activation of different signaling pathways such as JAK2/STAT3 and MAPK cascade/FOS. In the hypothalamus, LEP acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic and affects innate and adaptive immunity. Control of energy homeostasis and melanocortin production (stimulation of POMC and full repression of AgRP transcription) is mediated by STAT3 signaling, whereas distinct signals regulate NPY and the control of fertility, growth and glucose homeostasis. Involved in the regulation of counter-regulatory response to hypoglycemia by inhibiting neurons of the parabrachial nucleus. Has a specific effect on T lymphocyte responses, differentially regulating the proliferation of naive and memory T -ells. Leptin increases Th1 and suppresses Th2 cytokine production. Isoform A: May transport LEP across the blood-brain barrier. Binds LEP and mediates LEP endocytosis. Does not induce phosphorylation of and activate STAT3. Isoform E: Antagonizes Isoform A and isoform B-mediated LEP binding and endocytosis.
Protein Name Leptin Receptor
Lep-R
Hub219
Ob Receptor
Ob-R
Cd Antigen Cd295
Database Links Reactome: R-HSA-2586552 P48357-1
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Basolateral Cell Membrane
Isoform E: Secreted
Alternative Antibody Names Anti-Leptin Receptor antibody
Anti-Lep-R antibody
Anti-Hub219 antibody
Anti-Ob Receptor antibody
Anti-Ob-R antibody
Anti-Cd Antigen Cd295 antibody
Anti-LEPR antibody
Anti-DB antibody
Anti-OBR antibody

Information sourced from Uniprot.org

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