• Western blot analysis of lysates from COLO205 cells, using KIF11/Eg5 (Phospho-Thr926) Antibody. The lane on the right is blocked with the phospho peptide.
  • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using KIF11/Eg5 (Phospho-Thr926) Antibody
  • Western blot analysis of HELA KB SH-SY5Y 293T 3T3 lysis using Phospho-Eg5 (T926) antibody. Antibody was diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of COLO205 cells using Phospho-Eg5 (T926) Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-KIF11-Thr926 antibody (892-941 aa) (STJ90532)

SKU:
STJ90532

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Kinesin-like protein KIF11-Thr926 (892-941 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KIF11
Gene ID: 3832
Uniprot ID: KIF11_HUMAN
Immunogen Region: 892-941 aa
Specificity: Phospho-Eg5 (T926) Polyclonal Antibody detects endogenous levels of Eg5 protein only when phosphorylated at T926.
Immunogen: The antiserum was produced against synthesized peptide derived from the human KIF11/Eg5 around the phosphorylation site of Thr926 at the amino acid range 892-941
Function Motor protein required for establishing a bipolar spindle during mitosis. Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface.
Protein Name Kinesin-Like Protein Kif11
Kinesin-Like Protein 1
Kinesin-Like Spindle Protein Hksp
Kinesin-Related Motor Protein Eg5
Thyroid Receptor-Interacting Protein 5
Tr-Interacting Protein 5
Trip-5
Database Links Reactome: R-HSA-2132295
Reactome: R-HSA-6811434
Reactome: R-HSA-983189
Cellular Localisation Cytoplasm
Cytoskeleton
Spindle Pole
Alternative Antibody Names Anti-Kinesin-Like Protein Kif11 antibody
Anti-Kinesin-Like Protein 1 antibody
Anti-Kinesin-Like Spindle Protein Hksp antibody
Anti-Kinesin-Related Motor Protein Eg5 antibody
Anti-Thyroid Receptor-Interacting Protein 5 antibody
Anti-Tr-Interacting Protein 5 antibody
Anti-Trip-5 antibody
Anti-KIF11 antibody
Anti-EG5 antibody
Anti-KNSL1 antibody
Anti-TRIP5 antibody

Information sourced from Uniprot.org

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