• Immunohistochemical analysis of paraffin-embedded Human testis. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of 293 using p-Emt (Y512) antibody.

Anti-Phospho-ITK-Tyr512 antibody (478-527 aa) (STJ91164)

SKU:
STJ91164

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Tyrosine-protein kinase ITK/TSK-Tyr512 (478-527 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ITK
Gene ID: 3702
Uniprot ID: ITK_HUMAN
Immunogen Region: 478-527 aa
Specificity: Phospho-Emt (Y512) Polyclonal Antibody detects endogenous levels of Emt protein only when phosphorylated at Y512.
Immunogen: The antiserum was produced against synthesized peptide derived from the human ITK around the phosphorylation site of Tyr512 at the amino acid range 478-527
Post Translational Modifications Phosphorylated at Tyr-512 in the activation loop of the kinase domain by LCK. Subsequent autophosphorylation at Tyr-180 leads to the kinase activation. The autophosphorylated Tyr-180 lies within the substrate binding sequence of the SH3 domain. Ubiquitinated.
Function Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Required for TCR-mediated calcium response in gamma-delta T-cells, may also be involved in the modulation of the transcriptomic signature in the Vgamma2-positive subset of immature gamma-delta T-cells. Phosphorylates TBX21 at 'Tyr-530' and mediates its interaction with GATA3.
Protein Name Tyrosine-Protein Kinase Itk/Tsk
Interleukin-2-Inducible T-Cell Kinase
Il-2-Inducible T-Cell Kinase
Kinase Emt
T-Cell-Specific Kinase
Tyrosine-Protein Kinase Lyk
Database Links Reactome: R-HSA-202433
Reactome: R-HSA-2871809
Cellular Localisation Cytoplasm
Nucleus
Localizes In The Vicinity Of Cell Surface Receptors In The Plasma Membrane After Receptor Stimulation
Alternative Antibody Names Anti-Tyrosine-Protein Kinase Itk/Tsk antibody
Anti-Interleukin-2-Inducible T-Cell Kinase antibody
Anti-Il-2-Inducible T-Cell Kinase antibody
Anti-Kinase Emt antibody
Anti-T-Cell-Specific Kinase antibody
Anti-Tyrosine-Protein Kinase Lyk antibody
Anti-ITK antibody
Anti-EMT antibody
Anti-LYK antibody

Information sourced from Uniprot.org

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