• Western blot analysis of lysates from HeLa cells, HepG2 cells and HUVEC cells, using Integrin beta3 (Phospho-Tyr785) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-Integrin Beta 3 (Y785) Polyclonal Antibody diluted at 1:500

Anti-Phospho-ITGB3-Tyr785 antibody (739-788 aa) (STJ90684)

SKU:
STJ90684

Current Stock:
Host: Rabbit
Applications: IF/ELISA/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Integrin beta-3-Tyr785 (739-788 aa) is suitable for use in Immunofluorescence, ELISA and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IF ICC 1:50-300
ELISA 1:2000-20000
IHC-P 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ITGB3
Gene ID: 3690
Uniprot ID: ITB3_HUMAN
Immunogen Region: 739-788 aa
Specificity: Phospho-Integrin Beta 3 (Y785) Polyclonal Antibody detects endogenous levels of Integrin Beta 3 protein only when phosphorylated at Y785.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Integrin beta3 around the phosphorylation site of Tyr785 at the amino acid range 739-788
Post Translational Modifications Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding.
Function Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets. ITGAV:ITGB3 binds to fractalkine (CX3CL1) and acts as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling. ITGAV:ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling. ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling. ITGAV:ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling. ITGAV:ITGB3 binds to IL1B and this binding is essential for IL1B signaling. ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. ITGAV:ITGB3 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1. In brain, plays a role in synaptic transmission and plasticity. Involved in the regulation of the serotonin neurotransmission, is required to localize to specific compartments within the synapse the serotonin receptor SLC6A4 and for an appropriate reuptake of serotonin. Controls excitatory synaptic strength by regulating GRIA2-containing AMPAR endocytosis, which affects AMPAR abundance and composition. ITGAV:ITGB3 act as a receptor for CD40LG. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Herpes virus 8/HHV-8. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Coxsackievirus A9. (Microbial infection) Acts as a receptor for Hantaan virus. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Cytomegalovirus/HHV-5. (Microbial infection) Integrin ITGA5:ITGB3 acts as a receptor for Human metapneumovirus. (Microbial infection) Integrin ITGAV:ITGB3 acts aP05556s a receptor for Human parechovirus 1. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for West nile virus. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Protein Name Integrin Beta-3
Platelet Membrane Glycoprotein Iiia
Gpiiia
Cd Antigen Cd61
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-1566948
Reactome: R-HSA-210990
Reactome: R-HSA-2129379
Reactome: R-HSA-216083
Reactome: R-HSA-2173789
Reactome: R-HSA-3000170
Reactome: R-HSA-3000178
Reactome: R-HSA-354192
Reactome: R-HSA-354194
Reactome: R-HSA-372708
Reactome: R-HSA-4420097
Reactome: R-HSA-445144
Reactome: R-HSA-5674135
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cell Projection
Lamellipodium Membrane
Cell Junction
Focal Adhesion
Postsynaptic Cell Membrane
Synapse
Alternative Antibody Names Anti-Integrin Beta-3 antibody
Anti-Platelet Membrane Glycoprotein Iiia antibody
Anti-Gpiiia antibody
Anti-Cd Antigen Cd61 antibody
Anti-ITGB3 antibody
Anti-GP3A antibody

Information sourced from Uniprot.org

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