• Western blot analysis of lysates from COS7 cells treated with Serum 20% 15', using IRS-1 (Phospho-Ser323) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of HeLa cells, using IRS-1 (Phospho-Ser323) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IRS-1 (Phospho-Ser323) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of 293T using p-IRS-1 (S323) antibody. Antibody was diluted at 1:1000

Anti-Phospho-IRS1-Ser323 antibody (289-338 aa) (STJ91041)

SKU:
STJ91041

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Insulin receptor substrate 1-Ser323 (289-338 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IRS1
Gene ID: 3667
Uniprot ID: IRS1_HUMAN
Immunogen Region: 289-338 aa
Specificity: Phospho-IRS-1 (S323) Polyclonal Antibody detects endogenous levels of IRS-1 protein only when phosphorylated at S323.
Immunogen: The antiserum was produced against synthesized peptide derived from the human IRS-1 around the phosphorylation site of Ser323 at the amino acid range 289-338
Function May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
Protein Name Insulin Receptor Substrate 1
Irs-1
Database Links Reactome: R-HSA-109704
Reactome: R-HSA-112399
Reactome: R-HSA-112412
Reactome: R-HSA-1257604
Reactome: R-HSA-1266695
Reactome: R-HSA-198203
Reactome: R-HSA-201556
Reactome: R-HSA-2219530
Reactome: R-HSA-2428928
Reactome: R-HSA-2586552
Reactome: R-HSA-5673001
Reactome: R-HSA-6811558
Reactome: R-HSA-74713
Reactome: R-HSA-74749
Reactome: R-HSA-9603381
Reactome: R-HSA-9725370
Reactome: R-HSA-982772
Alternative Antibody Names Anti-Insulin Receptor Substrate 1 antibody
Anti-Irs-1 antibody
Anti-IRS1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance